SOIL INOCULATION. 2O9 



The plants used in the work included red clover, pea, bean, 

 vetch, and soja bean. The results obtained with each are 

 detailed below : 



RED CLOVER. 



The seeds were treated as above described. After four days, 

 when the sprouts were 34 to ^ inch long, those which were 

 to be grown with nitragin were immersed in a diluted culture 

 of the species desired (i c. c. nitragin to 20 c. c. distilled water), 

 after which they were planted 34-iiich deep in the sterilized soil, 

 in 6-inch pots. In the soil inoculated with tubercles, instead of 

 nitragin, the tubercles were placed, two or three together, 

 3^-inch deep by each seed. Eight seeds were planted in each 

 pot, and when the plants were well started they were thinned to 

 five in each. The pots were placed on a bench in the green- 

 house and a night temperature of 50° was maintained. 



In this trial 3 pots were planted without treatment, 3 were 

 given clover nitragin as above ; 3 pea nitragin ; 3 lupine nitra- 

 gin; 3 tubercles of Swainsona galegifolia and 3 tubercles of 

 horse bean (Vicia Faha). 



At this writing the plants present no marked differences. 



PEA. 



Preliminary treatment of soil and seed as for clover. The 

 plants were thinned to four in each pot when about three inches 

 high. The crop was harvested when in full bloom, as the plants 

 were all attacked with mildew and were losing their foliage. 



The results are given in tabular form on the following page. 



