^2 MAINE AGRICULTURAL EXPERIMENT STATION. I915. 



injection ibut in the first the syringe did not work well and 

 only a few cc. were given). 



Decem'ber i. Injected 1-2 gram of same powder mixed 

 with 10 oc. of 0.9 per cen/t. NaCl solution at 10.30 A. M. 

 Temperatures: — 11 iK. M., 102.0; 1.30 P. M., 101.2; 3.40 

 P. M., loi.o; 5.30 P. M., 100.4; December 2, 8.30 A. M., 

 101.6. 



December 5. Injected 1-2 gram of same powder in 15 

 cc. NaOl soiuit/ion suibcultaneoiusly ait 11. 15 A. ]\I. Cow 

 seemed to shoiw some rumfbling or difficulty in breathing 

 when we came to inject her. For this reason we used 

 subcutaneous route. Temperature just before injection, 

 II. 15 A. M., 102. 1 ; 12 N., 102.5; 2 P. AL. 102. 1 ; 5 P. M., 

 102. 1. 



Decemlber 9. Injected same dose subcutaneously at 

 11.30 A.M. 



Decemlber 10. Same dose subcutaneously at 11. 15 A. M. 



December 11. Same dose intravenously at 10.20 A. M. 



December 15. Injected 3-4 gram powder in 18 cc. 0.9 

 per cent. NalOl solution sulhoutaneously at 10.30 A. M. 



December 16. Injected 3-4 gra<m in 22 cc. 0.9 per cent. 

 NaOl solution; about half injected initravenously and half 

 subcutaneous ly . 



At this point the injections were stopped since the results 

 had been a'bsolutdly negative so far as concerned the sexual 

 functions of the cow. Of course the period included was too 

 short for any structural changes in secondary sexual characters 

 to have appeared. On the other hand, it was a sufficiently loiug 

 period for any effect on the aJbnormal sex-beihavior, the udder, 

 or the external genitals to have appeared. There was mot the 

 slightest change in any of these respects, either during or after 

 the period of the injectioins. 



Post-mortem Examination. 



On February 18, 19 14, the cow was slaughtered. She was 

 in good flesh and all the viscera were normal with the excep- 

 tion of the genital organs. The ovaries both showed extensive 



