STUDIES OF DISEASE PRODUCING SPECIES OF FUSARIUM. 221 



A fungus F VII isolated from diseased cucumbers in Maine 

 has agreed closely with these fungi in its growth. This was strik- 

 ing in the experiments testing the effect of different amounts of 

 several acids on growth. In each case these 3 strains were able 

 to grow in media containing larger amounts of acid than any of 

 the other 21 strains used in these tests. Two other species of 

 Fusarium have been obtained from diseased cucumbers. One 

 of these, F XX, shows some blue color in the mycelium while 

 in the other, F XIX the mycelium remains white. All of these 

 strains from cucumber produce large numbers of chlamydo- 

 spores in the cultures. These chlamydospores may occur singly 

 or several may occur together in the hypha. Two strains from 

 tomato, F XXI and F XXII, and one from squash, F XXIII, 

 agreed closely with the strain from cucumber in which the 

 mycelium remained white. 



In addition to the forms from potato which have been men- 

 tioned four others have been isolated. One of these, F 

 XXXVIII, which was isolated from sporodochia on stems has 

 showm the same cultural characters as F I froim apple, and the 

 fungus, F VIII, isolated from wheat in Maine. When apples 

 were inoculated with the potato fungus, rot followed. The other 

 three fungi illustrate very well the fact that a number of species 

 of Fusarium may occur in stored potatoes and that some of 

 these species. can be easily distinguished form one another by 

 their growth on a few culture media. One of these forms, F 

 XXXIII, showed blue color of mycelium, one red, F XXXV, 

 and the other white, F XXXIV, when grown on potato cylinders. 

 These fungi have been under observation for two years and 

 have not shown variation from these colors except in the case 

 of the red which has not been uniform. 



Each summer for the past three years specimens of China 

 aster, the stems of which were affected with a fungous disease, 

 have been sent to this laboratory. In each case cultures have 

 been secured by taking material from the inner part of the stem, 

 using sterilized instruments. The cultures secured from differ- 

 ent sources give evidence that the same fungus, F XIV, is 

 responsible for the disease in different localities. The fungus 

 shows a small amount of white aerial mycelium and no color 

 has appeared in the cultures. The spores are mostly of the non- 



