228 MAINE AGRICULTURAL EXPERIMENT STATION. I913. 



conidia were being produced. These were mostly of the obovate 

 and pyriform types, although the long type were not rare. 

 Cross walls were not seen in the long spores until after they 

 had separated from the conidiophores. 



Spores of F IV from apple, the greater part of which were 

 of the obovate type, were placed in hanging drops of distilled 

 water, potato broth, and dextrose broth at 3.30 P. M. At 8 

 A. M. the following day many stages of germination could be 

 observed. The obovate spores become somewhat swollen and 

 put out as a rule a single germ tube which soon branches. The 

 spores sown in distilled water germinated well but the growth 

 was soon checked while the mycelium in the broths made good 

 growth and spores were formed. 



Growth on Vegetable Cylinders. 



Cylinders of vegetables in tubes make excellent media for the 

 growth of the mycelium of species of Fusarium. At the present 

 time the writer has strains from 50 sources representing, how- 

 ever, a smaller number of species, all of which grow well on 

 cylinders of potato, beet, turnip and carrot. These media anj 

 especially the potato are used for keeping the stock cultures and, 

 in addition to this, series of cultures of the different strains 

 have been made in which a number of the fungi are grown on 

 the different vegetables at the same time for comparison. 



Potato Cylinders. All of the strains of Fusarium which have 

 been isolated here have been grown on this medium. The 

 amount of aerial mycelium and the color which develops are of 

 value in placing the forms from different sources ir, i?rge 

 groups. Part of these have produced a large amount of white 

 aerial mycelium with bright red color next to the potato, others 

 have shown a white mycelium with no other color except some 

 browning in old cultures, while others have developed blue color. 



The following fungi have shown a bright red color on this 

 medium. F I, F II and F IV from apples together with a 

 number of other isolations from apples which proved to be 

 identical with one or the other of these, all the forms with 

 obovate spores which were isolated from various hosts, F 

 rosemn/^ F XVIII, from wheat sent from Ohio, another Fusa- 



See foot-note p. 254. 



