232 MAINE AGRICULTURAL LXPERIMLNT STATION. 1913. 



by Smith and Swingle while the third should be placed in 

 another group of species. 



One Fusarium with white mycelium from squash, F XXIII, 

 and the two strains from tomatoes, F XXI and F XXII, have 

 resembled very closely in their growth the species from cucum- 

 ber in which the mycelium remained white. These fungi in 

 addition to chlamydospores produced larger rounded cells with 

 a small amount of contents. These cells were as much as 30 

 microns in diameter in some cases. 



Carrot Cylinders. The growth on this medium was so nearly 

 identical with that on beet cylinders that the details will not be 

 given. The most striking characteristic was the early develop- 

 ment and brightness of the red color in those strains which have 

 been described as showing this color on potato and beet. There 

 was much red color on this medium at the end of 3 days. 



Turnip Cylinders. All of the fungi made a good growth on 

 this medium. There was not so much development of the red 

 color on turnip as on potato, beet and carrot.' Microscopic 

 examination showed that the spore production agreed with that 

 on beet. 



Synthetic Agar. The species of Fusarium studied produce a 

 good growth of mycelium on this medium which is prepared 

 according to the following formula : 



Dextrose 50 g. 



Peptone 10 



Ammonium nitrate 10 " 



Potassium nitrate 5 " 



Magnesium sulphate 2.5 " 



Potassium monophosphate 2.5 " 



Calcium chloride o.i " 



Agar 20 " 



Distilled water 1000 c. c. 



This agar was titrated and made neutral with NaOH but it 

 was found that upon sterilizing changes took place which ren- 

 dered the medium somewhat acid. However, the medium which 

 has been treated in this way with NaOH is more satisfactory 

 for the growth of fungi than the same medium without this 

 treatm.cnt. 



