236 MAINE AGRICULTURAL EXPERIMENT STATION. I913. 



forms with obovate spores. Careful examination failed to 

 show the presence of spores. 



The forms from cucumber, tomato and squash produced 

 only a small amount of white aerial mycelium. One of the 

 species from cucumber, F VII, agreed with cultures of F. 

 oxysporum, F XV and F XVI, from potato in showing at the 

 center of each plate an area of reddish purple color. All of 

 the species from cucumber produced chlamydospores on this 

 medium. 



F. oxysporu'in, F XV and F XVI, from potato made good 

 growth on prune agar but did not produce much aerial 

 mycelium. When the cultures were one week old, the center 

 of each plate had taken on a reddish purple color for an area of 

 about 4.5 cm. in diameter. At three weeks old, the dark reddish 

 purple color had spread over the entire surfaces of the plates 

 and a small amount of white aerial mycelium had developed. 

 Examination showed large numbers of one-celled spores. Mate- 

 rial from near the center of one plate showed chlamydospores. 



Prune Decoction. This medium was prepared by cooking 6 

 large prunes in a part of the water, filtering the decoction and 

 making up the filtrate to 1000 c. c. The fungi were grown in 

 tubes containing 5 c. c. of this material. All of the forms which 

 were tested made some growth but this was not a favorable 

 medium for normal development. The mycelium spread 

 through the liquid and in many cases formed a pellicle on 

 which a small amount of aerial mycelium developed. Material 

 from cultures of different ages was examined and it was found 

 that few spores were produced and that these were abnormal 

 as compared with spores of the same fungus grown on more 

 favorable media. 



Sugar Broths. In order to determine whether the growth in 

 media containing different sugars would bring out character- 

 istics which might be used to assist in classification, beef ex- 

 tract broths were prepared each of which contained one per 

 cent of a different sugar and each of the strains, F I to F 

 XXIV, was grown in the different media at the same time for 

 comparison. The sugars used were dextrose, saccharose, lac- 

 tose, and mannite. It was found that all the strains made good 

 growth of mycelium in each broth but that dextrose and sac- 

 charose gave better growth than the others. None of the cul- 



