244 MAINE AGRICUIvTURAL I5XPE;RIME;nT STATION. 1913. 



plants were available so the number of buds used in the earlier 

 experiments was necessarily small.' 



Two buds of the Enchantress variety were inoculated March 

 4, 1910, with material of the apple fungus, F IV. The plants 

 were placed under bell-jars and were kept covered for three 

 days. At the end of this time it could be noted that the fungus 

 was making some growth at the points of inoculation. The buds 

 did not increase much in size and 12 days after inoculation the 

 calyx of each bud appeared somewhat discolored. One bud was 

 examined and it was found that all parts inside the calyx were 

 destroyed by the fungus. When material from the decayed 

 petals was teased out on a slide and examined with a micro- 

 scope, spores of both the obovate and septate types were found, 

 although the number of either kind was not great. The outer 

 covering of the other bud was removed with instruments steril- 

 ized by flaming and the inner decaying part was placed in a 

 tube containing 5 c. c. of sterile distilled water. White aerial 

 mycelium grew out from this and the second day large numbers 

 of spores were being produced, a part of which were obovate 

 and a part of the long septate type. After 5 days the mycelium 

 formed a thick red pellicle over the surface of the water. The 

 white aerial mycelium was bearing many spores of the obovate 

 and pyriform types which agreed in size with the spores of the 

 carnation bud rot fungus. 



April 21, 1910, 3 Enchantress buds were inoculated with 

 F IV from apples. One of these buds was removed and exam- 

 ined 4 days later when the fungus showed only a small amount 

 of growth. Two days later one of the other buds was examined 

 and it showed that the fungus was making considerable growth 

 in the tissues. April 29, 8 days after inoculation, the third bud 

 was examined. All parts inside the calyx were destroyed and 

 spores of the fungus were found. It is probable that if the first 

 2 buds had not been examined until 8 or lo days after inocula- 

 tion they would have shown a greater amount of decay as the 

 fungus was growing in each at the time of examination and it 

 has been found that about 10 days are necessary under favoraJble 

 conditions for the complete destruction of the buds. 



On account of the similarity in cultural characteristics of the 

 fungi with obovate spores and certain species of Fusarium it 

 was decided to test a number of fungi which gave evidence of 



