STUDIES 0]? DISEASE PRODUCING SPECIES OF EUSARIUM. 245 



being typical species of Fusarium to see if these alsv would 

 cause decay of carnation buds. 



April 20, 1910, 3 buds of Enchantress were inoculated with 

 F I from apple and check punctures to which no fungus material 

 was added were made in 2 buds. Five days later one of the 

 buds, which was large at the time of inoculation, had opened 

 and showed no decay. April 27 both of the others showed all 

 inside the calyx destroyed and large numbers of spores of the 

 fungus used in making the inoculations were present. One of 

 the check buds was entirely open April 30 and showed no decay, 

 the other opened a few days later and showed no injury from 

 the puncture. 



Since F I had caused decay another typical culture of 

 Fusarium later found to be identical with F I was tested. April 

 28, 4 small fbuds were inoculated with F III from apple. One 

 week later one of these was examined and it showed a small 

 amount of decay of the petals and stamens. May 9 two other 

 buds were examined and they showed more decay than was 

 noted in the one examined May 5. When the last bud was 

 examined, May 11, the interior was badly decayed. 



May 5, 1910, 4 buds were inoculated with F III. May 16, 

 3 of the buds were badly rotted while the fourth remained 

 healthy. 



Five Enchantress buds were inoculated with F IV May 5 

 and were all badly rotted one week later. Three buds inocu- 

 lated with the same fungus May 18 were decayed at the end of 

 one week. 



May 12, 1910, 2 Enchantress buds were inoculated with each 

 of the fungi, F I, II and IV, from apple. All of these 6 buds 

 were destroyed by the fungi within 10 days. 



F XII, the organism carried as F. pittrefaciens, is in every 

 respect a typical species of the genus. July 11, 1910, 26 En- 

 chantress buds were inoculated with this fungus. Eight days 

 later, 21 of the buds were badly rotted. Material from a part 

 of these was examined and septate spores of the Fusarium were 

 found with no evidence of the presence of any other fungus. 



July 26, 1910, 12 buds were inoculated with F XII and punc- 

 tures were made in 13 buds to serve as checks. The injuries to 

 the check buds were exactly like those in the others except that 

 no fungus material was placed in the wounds. Examination ii 



