STUDIES OF DISEASE PRODUCING SPECIES OF FUSARIUM. 247 



In 191 1 tests were made of part of the fungi which had been 

 used in 1910 on 2 other varieties of carnation, Lady Bountiful 

 and Windsor, and a number of fungi which had not been used in 

 1910 were tested on these varieties as well as on Enchantress. 



May 4, 191 1, yj Enchantress buds were inoculated with F II 

 from apple and punctures were made in 22 buds as checks. May 

 22, 20 of the inoculated buds showed decay and one of the 22 

 checks had become infected and was also rotted. 



May 24, 191 1, 30 Enchantress 'buds were inoculated with F 

 II from apple and 37 with the organism carried as P roseum, 

 F XVIII from wheat. Three buds of Lady Bountiful and 5 of 

 Windsor were inoculated with F II from apple. June 6, 10 days 

 after inoculation, 24 of the 30 buds inoculated with F II showed 

 rot, all of the Lady Bountiful had rotted, 3 of the 5 Windsor 

 had decayed. Of the 37 buds inoculated with F XVIII, 25 were 

 destroyed by the fungus. 



June 12, 1911, 24 buds were inoculated with material of the 

 fungus, F XXIV, from June grass from a bean pod culture 3 

 days old, 22 buds with material from a potato cylinder culture 

 5 days old of the fungus, with obovate spores, F IX, from 

 potato, 15 buds with material from a potato cylinder culture 5 

 days old of the fungus, F XIII, from sunflower, and check 

 punctures were made in 10 other buds, June 21, 9 days later, 

 22 of the 24 buds inoculated with the fungus from June grass 

 were decayed and all of the 15 buds inoculated with the fungus 

 from sunflower were destroyed by the fungus. None of the 22 

 buds inoculated with the fungus from potato and none of the 10 

 checks showed any rot. 



The fungus, F XXVI, isolated from quack-grass was used 

 July 7, 191 1, in making inoculations of 7 Enchantress, 6 Lady 

 Bountiful and 12 Windsor buds. July 21, 6 of the 7 Enchan- 

 tress, 5 of the 6 Lady Bountiful and 10 of the 12 Windsor were 

 rotted. Examination of material from some of these showed 

 the presence of many spores of both the septate and obovate 

 types. 



Eleven Enchantress, 7 Lady Bountiful and 12 Windsor buds 

 were inoculated July 25 with material of the fungus, F XXV, 

 from red top. Two weeks later, 8 of the 11 Enchantress, 5 of 

 the 7 Lady Bountiful and 9 of the 12 Windsor were decayed. 



