STUDIES OF DISEASE PRODUCING SPECIES OF FUSARIUM. 249 



to the carnation bud might be open to question. However, this 

 point seems to have been determined rather definitely by inocu- 

 lating a number of buds of the same variety with very similar 

 fungi from different sources at the same time. The experiment 

 of June 12, 1911, will illustrate this. Th,e fungi used were the 

 strain from June grass, F XXIV, which seems to be identical 

 with S. poae as described by Stewart, the fungus with obovate 

 spores from potato tuber, F IX, and the fungus from sunflower, 

 F XIII. The material for all of the inoculations was taken 

 from young actively growing cultures. Nine days after inocula- 

 tion 22 of the 24 buds inoculated with the fungus from June 

 grass and all of the 15 buds inoculated with the sunflower 

 fungus were decayed. None of the 22 buds inoculated with the 

 potato fungus showed any rot. Since the carnations were all 

 of the same variety, Enchantress, and the different lots were 

 growing side by side in the greenhouse, the fungus from potato 

 had the same conditions for the attack of the buds as the other 

 2 fungi and the only conclusion which the writer can reach from 

 the results is that the patoto fungus is not parasitic on carna- 

 tion buds. This fungus resembles the carnation bud rot fungus 

 more closely than do a number of other fungi which caused a 

 rot of the buds. The fact that 2 other strains which are so 

 like the fungus from June grass that it is impossible to distin- 

 guish cultures of one from the others failed to cause the rot of 

 the buds would seem to indicate that certain strains are unable 

 to go from one host to another. The most striking example of 

 this was the fungus from fowl meadow grass, F XXVII. This 

 fungus seemed identical in cultural characters with the strains 

 from other grasses and yet it failed to cause the rot of carna- 

 tion buds while the other strains caused the rot in a rather large 

 proportion of the inoculations. 



In his first account of carnation bud rot, Pleald regarded a 

 species of Fusarium as the cause of the disease. In his later 

 publication, the fungus described was identical with the one 

 described by Stewart as Sporotrichum poae and it was regarded 

 as S. anthophiluni which Stewart proved to be the same as 

 S. poae. The inoculation experiments reported in this paper 

 prove conclusively that a number of typical species of Fusarium 

 cause a rot of carnation buds upon inoculation. Therefore, it is 

 possible that Heald worked with different fungi, one a typical 



