192 MAINE) AGRICUIvTURAIv EXPERIMENT STATION. I909. 



from the point of infection or were on the border Une between 

 the decayed and the undecayed tissue with a scalpel which had 

 been sterilized by heat and transferring to plates of agar. In 

 no case has Coryneuin foliicolum been isolated from a naturally 

 infected apple. 



In order to test the ability of the fungus to cause decay, two 

 ripe apples were inoculated October 31, 1908. Only a very 

 small amount of decay developed but that this decay was caused 

 by Coryneum was proved by reisolating the fungus in pure cul- 

 ture from the decaying tissue. 



Six green apples were inoculated August 24, 1909. The 

 fungus grew to a slight extent at points of inoculation but did 

 not spread into surrounding tissue to cause decay. 



Three ripe apples were inoculated October 5, 1909. A slow 

 decay took place. At the end of two weeks the decayed region 

 was about 1.5 cm. in diameter, and increased very slowly after 

 that time. 



Three ripe pears were inoculated September 16. There was 

 a little growth at the points of inoculation but the fungus did 

 not spread to cause much decay. 



These inoculations show that Coryneum can cause a slow 

 decay of ripe fruit but when the decay caused by this fungus 

 is compared with that caused by such fruit decaying fungi as 

 Sphccrofsis and Penicillium it is seen that Coryneum is not of 

 much importance as a fruit ddcay. 



CULTURAL STUDIES. 



When the spores of Coryneum foliicolum from either leaf- 

 spot or canker are placed under favorable conditions for growth 

 they germinate readily. In hanging drops of sterile, distilled 

 water nearly all of the spores had germinated at the end of 16 

 hours at 65° to 70" F. The cells become swollen and rounded 

 and germ tubes are produced from one or more cells as shown 

 by Fig. 35. About the same characters are shown by spores 

 germinated in hanging drops of prune decoction. When spores 

 are sown in dilution plates of prune or bean agar, the germ 

 tubes have begun to branch by the end of 24 hours and in 4 or 

 5 days the mycelium has begun to produce spores after the 

 manner of the Hyphomycetes as shown in Figures 2'j, 33 and 

 34. If this fungus were classified according to its characters. 



