iQo Maine; agricuIvTural experime^nt station. 1909. 



on the dead bark. Some sections of these pycnidia were pre- 

 pared and from one of these sections Fig. 42 was taken. The 

 pycnidia develop in the bark and when they are mature the 

 outer layers of the bark are ruptured and the pycnidia appear 

 above the surface. 



Two more young trees were inoculated Alay 18 in the same 

 way and gave practically the same results. 



In order to test the parasitism of the fungus on old trees in 

 the orchard, 12 places were inoculated June 4. In some cases, 

 an inoculation was made in a branch which had been inoculated 

 with Coryneum. This fungus did not form as well marked 

 cankers on these branches as Coryneum but showed that it was 

 able to grow as a parasite. Eleven places were inoculated on 

 branches of Ben Davis and Baldwin trees in the orchard June 

 24. All but one of these places developed small cankers. 



Attempts were made to infect apple leaves with spores from 

 pycnidia from bean pod cultures using the same methods which 

 were used with Coryneum. In no case was there any evidence 

 that the fungus attacked the living leaves. 



It has already been shown that this fungus causes a decay 

 of ripe apples. It is well known that some of the fungi which 

 cause decay of ripe apples cannot cause decay of green fruit. 

 To determine the effect of this fungus, 8 green apples were 

 inoculated August 10, 1909. The fungus grew at the points of 

 inoculation but the decay did not spread to a great extent in 

 any of the apples except one. In this apple, some other fungus 

 may have assisted in the decay but the pycnidia of the fungus 

 with which it was inoculated appeared on the surface. The 

 other 7 apples showed at the end of one month a small decayed 

 region about .5 cm. in diameter as illustrated by Fig. 25. 



In September and October, 1909, inoculations of ripe pears 

 and of apples which were ripe but not over-ripe were made 

 using material of the fungus which had been carried in culture 

 for more than a year. The pears decayed rapidly and the 

 fungus mycelium broke out over the surface of the decayed 

 part. After 10 days, pycnidia in large number were found 

 among this mycelium. The decay of the apples took place at 

 about the same rate that has been described for the apples 

 inoculated in 1908. 



