THE CHINCH BUG TN MAINE. 49 



Experiment 2. Seven long-wingQd and five short-winged 

 forms were frozen in a block of ice from January 17th to 23rd. 

 No signs of life were shown after thawing out. 



Experiment 3. Fifteen long-winged and eight short-winged 

 forms were packed in snow and frozen from January i8th to 

 23rd. After thawing out two long-winged and four short- 

 winged forms walked about actively ; the rest did not revive. 



Experiment 4. Ten long-winged and six short-winged forms 

 were frozen in an open box for fifteen hours. Upon thawing 

 out two gave no signs of life. After being kept for nine hours 

 at a temperature of 65° the fourteen surviving bugs were refrozen 

 for fifteen hours and then thawed out, when five long- winged 

 and three short-winged forms revived. After nine hours at a 

 temperature of 65° they were refrozen for fifteen hours, during 

 which time the minimum temperature sank to 16° below zero. 

 When thawed out all revived, but during the following nine hours 

 at 65° temperature the three short-winged forms and two of the 

 long-v/inged ones died. The remaining three long-winged forms 

 were then again refrozen for fifteen hours, after which none 

 revived. 



Experiment 5. Thirty-two long-winged and two short- winged 

 forms taken from beneath a log on January 23rd were kept at 

 the ordinary temperature of the laboratory in a covered paste- 

 board box. Several thicknesses of filter paper were laid on the 

 bottom of the box and well moistened twice daily for eight days, 

 when only two bugs remained alive. 



Experiment 6. Fifty-three short-winged and fifteen long- 

 winged bugs picked up from the ice on January 23rd were kept 

 at laboratory temperature in a covered box with filter paper 

 moistened twice daily for eight days. Nineteen long-winged 

 and eight short-winged forms were alive at the end of that time. 

 These twenty-seven were continued at the same temperature but 

 without moisture for seven days longer when five short-winged 

 forms still remained alive. 



Experiment 7. Three long-winged forms were frozen for 

 twenty hours in a dry glass tube. None revived when thawed 

 out. 



Experiment 8. Three long-winged forms were frozen for 

 twenty hours in a block of ice. None revived after being thawed 

 out. 5 



