ACUICLM-TUKAL EXPKKIMKNT STATION. 207 



By using watch-glasses ground together and clamped the u?e of 

 :a desiccato is avoided unless the weighing is to be deferred for 

 .quite a tinae. 



.Fat. 



The portion dried for solids is removed from the watch-glass, the 

 'tinfoil drawn to a thimble lorm around the asbestose, perforated 



with a pin, placed in the extracter, the fat extracted with ether and 



dried for one hour at 100° C. and weighed, 



.Nitrogen. 



About two grams of milk are run into the flask from a weighing 

 pipette and without drying down the nitrogen is determined by 

 Kjeldahl method using only 10 c. c. sulphuric acid. Heat should 

 be applied very gently and the flask shaken frequently until the 

 -water is boiled out, otherwise a loss of nitrogen may occur from the 

 milk spattering on the hot walls of the flask and burning off. 



Ash. 



Two grams are ignited at a low red heat in a platinum dish till 

 constant weight is obtained. 



SUGAR AND STARCH IN COARSE FODDERS. 



Sugars. 



Five grams of the finely powdered substance are placed in a 

 •beaker, 100 c c. water at 40*^ C added and allowed to stand for 

 one-half hour, with frequent shaking. The extract is then filtered 

 through paper into a 250 c. c. flask, the substance brought upon the 

 iiiter and leached with water at ordinary temperature till the solu- 

 tion is made up to 250 c. c. 



Twenty-five c. c. of this solution are taken for determination of 

 glucose and another portion of twenty-five c. c. is heated, after 

 fiddiug a few drops of HCl, in a covered beaker on the steam bath, 

 for one-half hour, then neutralized with IvHO and total sugar 

 determined. 



Allihn's method and tables* were used in all cases for all sugar 

 determinations, except that instead of reducing the copper oxide to 

 copper it was ignited and weighed as CuO and the Cu calculated. 

 Several comparisons showed this mtthod to be accurate. 



*Freseneus's Quan. Cliem. Analysis, 6th Ed., p. p. 595-59". 



