30 



ARON. 



can be no doubt that fat has been lost, since the changes in the general 

 appearance of the animals are those which we regard as characteristic of 

 a loss of fatty tissue. 



The quantity of protein in the muscles of dogs II and III (experi- 

 ment I) expressed as percentage of the live weights is slightly lower than 

 the percentage of muscle protein in dog IV. The reduction in fat is 

 considerably greater, the amount of this constituent, also calculated as 

 a percentage of the live weight, in dogs II and III is only one-half the 

 quantity which it is in dog IV. If we add the quantities of bone-ash, 

 the protein, and fat of the muscles and bones, there remains of what 

 I call the "body," a residuum consisting almost entirely of water, muscle- 

 ash, and perhaps glycogen. This residuum amounts to approximately the 

 same percentage (74 per cent) in the three animals. 



Experiment III also gives infonnation concerning the constituents 

 of the "organs." The protein in the organs is not diminished in dog 

 XI kept on a low ' diet, as compared with the other two dogs. The 

 protein of the muscles, on the contrary, is greatly diminished. The fat 

 varies somewhat in these animals. They did not receive the same 

 quantities of food, and were not entirely alike, but it is clearly seen that 

 number XI shows a considerably lower content of fat than do numbers 

 VIII and XII. Fat, therefore, is not only lost from the "body," but 

 also from the organs. 



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Isli of Prtten Pmein fat 

 luits tjbtnis cjmiida 



Orpn Badj Orjan Haic/e Bime 8iafts» 

 Fit fjt Proteii fntm Pnttin 



DiAGEAM 1. — Composition of dogs II to VI of Experiments I and II. 



