32 



ARON. 



of dog number VI contained about one-fourth as much protein as that 

 . of dog V and of normal dogs (18 to 20 per cent) . 



The muscles contained only one-half of the normal amount of tot-al 

 solids/* The water content of the brain had also increased considerably. 

 It will be remembered that the weight of the fresh brain of dog VI was 

 57 grams as compared with 53 grams for that of the full-gi'own control 

 dog V. However, the water content was so much greater that the 

 amount of total solids in the brain of dog VI was in reality smaller than 

 that of number V. This process of hydration (Verwasserung) brings 

 about the condition in which the weight of the brain of a dog kept at 

 low diet is gi'eater than that of a normal control animal. 



Blood Brain 6 



ones 



*1uscles 













































?• 









W/. 







ft 





a 



Wa 

































1 



v/M 



m 































W/, 





W/. 



m 



M 





V yi vvi 



vvi 



m 



Sohds 



Water 



Diagram 2. — Percpntage of water and solids in blood, brain, bones and muscles of dogs 



V and VI of Experiment II. 



The bones also show an increased water content in dog VI, as compared 

 with dog V. However, in this case the percentage of ash in the bones 

 is not diminished, and that of protein only very slightly so. Water 

 in the starved bone really takes the place of fat in the normal one. These 

 conditiqns are shown in diagram III. 



The replacement of body substances by water in the different parts 

 of the body, and the almost complete destruction of fat, demonstrates 



^* For this determination 25 grams of lean muscle from corresponding parts 

 of the leg were selected, weighed and dried. The quantity of air-dried muscle 

 was determined, then the dried material was powdered and the water content of 

 this powder determined. 



