94 BOYNTON. 



which had died very suddenly in the vicinit}- of Taytay. Mr. Cattell 

 stated that he made the smear immediately after the death of the animal. 

 The staining material at hand was not of the best kind for distinguishing 

 anthrax. However, upon microscopic examination, there was observed 

 a large number of rather long, rod-shaped organisms with square-cut 

 ends, some of them in chains, and others single, many being surrounded 

 by what appeared to be capsules. It hardly seemed probable that these 

 bacteria were putrefactive organisms, since the preparation was taken 

 from the animal soon .after death, but to be sure of the fact several 

 guinea pigs were sent to Taytav, and instructions were given to the effect 

 that if any animals there died in a similar manner a guinea pig should 

 be inoculated with some of the blood, smear preparations made, a piece 

 of the ear of the dead animal cut off, and both ear and smear preparations 

 sent to the writer's laboratory at the Bureau of Science in Manila. 



Case I, number 17. — Carabao owned by Juan Villanueva, Santa Ana, Taytay, 

 Rizal; died August 30, 1910. One guinea pig inoculated at 2.40 p. m., August 

 30, with blood taken from this animal after death ; two smear preparations and 

 a piece of the ear of the dead carabao were received on the morning of August 31. 



The smear preparations were stained with an aqueous solution of methylene 

 blue, recommended by M'Fadyean.'' A large number of rod-shaped organisms 

 with square-cut ends, occurring singly, in pairs and in chains, were present. 

 Practically every organism was surrounded by an amorphous, violet or reddish- 

 purple granulai' material. M'Fadyean states that he has never found this reaction 

 in animals dead from diseases other than anthrax. 



Smear preparations were made from blood procured from the ear. These 

 smears were stained in a similar manner, and similar results were obtained. 

 Agar cultures were made from blood obtained from the ear. 



September I:, Guinea pig was found dead in the laboratory in the morning. 

 Smear preparations made from the spleen, liver, and heart blood showed the 

 presence of anthrax bacteria in stained preparations. Frozen sections made of 

 the kidney, stained with carbol-fuchsin, showed the presence of anthrax bacteria 

 in the capillaries between the tubules and in the glomeruli. Agar cultures were 

 made from the spleen, liver, and heart blood. Agar culture made from the ear 

 showed almost pure culture of anthrax bacteria characterized by the ground- 

 glass appearance along the edge of the colonies, and showed, under the low poM'er 

 of microscope, long flexible filaments combining to form thread-like bundles. 



September 2: Agar culture made from spleen, liver, and heart blood showed 

 pure cultures of anthrax. An emulsion of some of the cultures obtained from 

 the spleen was made in sterile water, and 1 cubic centimeter of this was injected 

 subcutaneously into a guinea pig. 



September 3: Giiinea pig was found dead in the morning. Smear prepara- 

 tions from the spleen, liver, and heart blood showed the i^resence of large numbers 

 of anthrax bacteria. Agar cultures were made from these organs. 



September J/ : Agar cultures made from spleen, liver, and heart blood showed 

 pure cultures of anthrax bacteria. 



Juan Villanueva owns another carabao, which, up to September 14. appeared 



' A Peculiar Staining Reaction of the Blood of Animals Dead of Anthrax. 

 Jotirn. Comp. Path, d Therap. (1903), 16, 35. 



