96 BOYNTON. 



Case IV, number 25 — Carabao owned by Pedro Banals, San Gaibro, Taytay, 

 Rizal, died September 13, 1910. The ear of the carabao was received in the 

 laboratory early in the morning of the 14th. In smear preparations from the 

 blood of the ear, stained with aqueous methylene blue, no rod-shaped organisms 

 could be found. The only organisms seen were a very few micrococci. Agar 

 cultures were made of blood from the ear. 



September 15: Agar cultures showed no anthrax colonies, there being a few 

 scattered colonies which resembled Micrococcus pyogenes aureus. Cover-glass 

 preparations from several colonies stained with aqueous methylene blue ' showed 

 the presence of a micrococcus. As far as can be decided from smear preparations 

 and cultures, it would appear that this animal did not die of anthrax. 



This man owns three more carabaos. These and the dead one were pastured 

 every day in a pasture called Mapandon, with many other carabaos. He bought 

 the carabao which died in San Mateo, Rizal, about three months before. 



Case V, number 32. — Carabao owned by Rufino del Rosario, Mapondon, Tay- 

 tay, Province of Rizal; died on the evening of September 28, 1910. 



The ear of the carabao received in the laboratory early in the morning of the 

 29th. Smear of blood from the ear stained with aqueous methylene blue showed 

 presence of anthrax organisms giving M'Fadyean's reaction. Agar cultures were 

 made from blood of ear. 



September 30:. Agar cultures from ear gave practically pure cultures of anthrax. 



This man owns five more carabaos, which, with the one that died, were pastured 

 every day with many other carabaos, in Mapandan, a pasture ground situated on 

 the opposite side of the town from Libis. 



Case VI, number 33. — Carabao owned by Mariano de los Reyes, San Isidro, 

 Taytay, Rizal, died on the morning of September 29, 1910. 



The ear of the carabao was received in the laboratory on the morning of the 

 30th. Smears of blood from the ear, stained with aqueous methylene blue, showed 

 presence of anthrax organisms giving M'Fadyean's reaction. Agar cultures were 

 made from blood of ear. 



October 1 : Agar cultures from ear gave practically pure cultures of anthrax. 



This man owns two more carabaos. These and the dead one were pastured 

 every day with many other carabaos in a pasture called Lambac, a subdivision of 

 Libis. He had owned this carabao several years. 



Case VII, number 39. — Carabao owned by Lazaro del Valle, barrio of San 

 Isidro, Taytay, Rizal, died October 27, 1910. 



The ear and two blood smears from the carabao were received in the laboratory 

 on the afternoon of the 27th. Blood smear stained with aqueous methylene blue 

 sho\ved presence of anthrax organisms giving M'Fadyean's reaction. Agar cul- 

 tures were made from blood of ear. 



October 28:. Agar cultures showed practically pure culture of anthrax. 



This carabao was pastured on the 14th and 15th of October in Libis, but 

 from the 15tli of October to the time of its death the carabao was pastvired in 

 the fields of the barrio of San Isidro. 



CONCLUSION. 



1. From the results derived from the cultures^ staining reaction, and 

 animal inoculations, it is evident that anthrax exists in the vicinity of 

 Taytay. 



