Hfp 



'e „ S^ 



^^-as also "" 



be 



come 



'^' and the 

 """^ ^^'^s found to 



'"^ «f the floid ,;; 



orulcE 



% 



were 



J 



:tive. 



t}'-eight hours showJ 

 '■0 hours the turbilt)' 

 t amount of sedimel 

 he next twentj' 

 .eemed to undergo 

 in the warm bath 

 :d. Its odour was 

 .ked turnip. Mm 

 3Ugh their movemetli 



pvi-xxi). 



,V and ils ^^ 



/ had huon^ 



i 



Lsion 



hange- 

 forty 



m 



tffentf 

 In tb< 



,eigW^«^^'; 

 , the flask wa« 



op 



ti 



APPENDIX C. 



XXXV 



microscopically, it was found to contain multitudes of very 

 lano-uid Bacteria. 



No. XVII. Neutralized Infusion of Turnip + | gr. 

 of Cheese ^ in thirty-six hours showed a well-marked 



pellicle 



When 



opened, after seventy-two 



hours, there was a violent outrush of gas, though the fluid 

 was still neutral. Portions of the thick pellicle were found, 

 on microscopical examination, to be made up of Bacteria, 



F 



Vibriones, and an abundance of long, interlaced Leptothrix 

 filaments. Bacteria also existed abundantly in the fluid, 

 though their movements were very languid. 



c. Fluid in a Bent-neck Flask^ having Eight acute Flexures. 



No. XVIIL Simple Turnip Infusion after forty-eight 



hours showed no change. It was kept in the warm-bath 



for twelve days, and during the whole of this time the fluid 



remained quite clear. The tube was then broken i^ inch 



above the bulb (which was re-immersed in the bath), leaving 



the fluid exposed to the air through the straight open tube. 



The fluid at this time was odourless, and its re-action was 

 still faintly acid. 



The infusion remained thus exposed for six days without 

 undergoing any apparent change. On the eighth day a very 

 slight whitish sediment was noticed, which had increased in 

 quantity by the tenth day, though there was still no trace of 

 general turbidity. On the eleventh day some of the sediment 

 examined in a drop of the fluid, and it was found to be 

 wholly composed of rather large TorulcE cells — the largest 

 toeing about -^oV/' in diameter, though all the smaller sizes 

 ^vere abundantly represented. Not a single Bacterium or 



Til '^^^ filtered infusion of turnip was neutralized by liquor potassse. 

 2^^heese (Cheddarj was new and not in the least mouldy. 

 The fluid itself being somewhat opaque, the first stages of increased 

 ™idity from presence of Bacteria could not be detected. 



€2 



was 



\, 



