J h 



^/^^. 



'^^* Of C 



APPENDIX C. 



xli 



\ 



hi 



h. 



Us nect 



quite 



tile 



I 





'"^ " *' 1% 



tion ; the fluid was found to be devoid of all odour, it had a 

 slightly bitter taste, and its re-action was very faintly acid. 

 On microscopical examination no living things were found ; 

 there were no Bacteria^ no Vibriones^ and no Torulix, only 



some mere granules, a small amount of amorphous matter, 

 and a few fibres \ 



No. XXXIV. 



Neutralized bv Am 



^^e but V 



^'^7 acfo 



^^ght acuk Fkx 



mi 



monic Carbonate in forty-eight hours showed a slight tur- 

 bidity, which slowly increased during the next two days. In 

 two days more the turbidity was very great, and there was 



r 



also a considerable amount of sediment. The fluid was then 

 examined microscopically, and found to contain myriads of 



fusion showed no * large but very languid Bacteria. 



in the warm bal 

 clear, the tube ^i-as 

 from the bulb; ani 

 h. After three te 

 s boiled in the 

 the steam was quite 

 i in the water^ 



(still 



Flask whu 

 Ebullition 



No. XXXV. H 



underwent no apparent 



w 



•ith tlie necl 



:d bending) w 

 initted to examlM- 



5, 



to those 



met wtl * 



.,, No. 36> P' '^^' 



«■ 



change for the first twelve days, then (the bulk of the fluid 

 still remaining clear and bright) small greyish-white flocculi 

 began to collect at the bottom of the flask, which very 

 slowly increased in quantity during the succeeding twelve 

 days. At the expiration of this time the flocculi were pretty 



the fluid was otherwise bright. The 



numerous, 



though 



th ^f-i.^^^^'l 

 ferments 



table fluid; ^; 



¥ 



less 



ed jn^': 



% 





jiisio" 



fett' 



turbid in thirty-six hours, and was then found to contain multitudes of 

 Bacteria ; and the characteristic odour of the turnip infusion was still 

 appreciable. 



The results of this experiment are most interesting, especially if 

 compared with that which takes place W'hen some of the same fluid is 

 ^eulralized by ammonia carbonate (No. XXXIV.), or when a similar 

 ™d (as in No. XXX.) is contained in a flask sealed during the ebulli- 

 tion of the fluid, or with what occurred in Nos. XIII. and XXXII. 



^ the present case the second boiling seems to have destroyed what 

 small amount of fermentability there was still remaining in the solu- 

 "on; but in No. IX, fermentation did take place after the second 



oiling— though it occurred only under the influence of diminished 

 pressure and a higher temperature. 



