■ ^'"e. 



^> nowpv 



'\vever 



'C chai 



existed i. 



'^^^' and a ,: 

 some of,. 



'■SlI 



% 



^; though in rhk.; 



>urth day the 

 ^^re were no 

 oscopically, the U 



y 



JfeutralizedbyAin- 



was decidedly tui, 

 3re marked, and tta 

 fortv-eisht hours ta 



-eased. Ontheta 

 containing an abi^- 



,hich when the hi 

 up principally of ' 



XVIII 





-xlvh; 



hours 



nten 

 vht ho^^' 



-ei 



tills ^ 



0' 





o jjje su 



>llicle on 



use 



d (^^ 



alte 



red) 



in 



APPENDIX a 



xliii 



a 



and, in addition, a small amount of deposit. On examina- 

 tion, the fluid and the pellicle were found to contain an 

 bundance of Bacteria^ Vibr zones, and Leptothrix filaments. 



No. XXXIX. TTirnip Infusion + ^V of Carbolic Acid, 

 after eight days showed no appreciable alteration in appear- 

 ance S no trace of pellicle or deposit. When examined 

 microscopically, however, the fluid was found to contain 

 some very minute Bacteria^ though they were by no means 

 abundant. 



r 



No. XL. Hay Infusion had become quite turbid in 

 twenty -four hours, and several shades lighter in colour. 

 After forty-eight hours the colour of the infusion was stiU 

 lighter ; there was more turbidity, and some sediment. On 

 microscopical examination, the fluid was found to contain 

 an abundance of Bacteria^ Vihriones^ and short Leptothrix 

 filaments. 



+ ^V of Carbolic Acid, 



No. XLI. Hay Infusion + ^V 



showed no apparent change^ after forty-eight hours, and 

 when examined microscopically it revealed no trace of 

 Bacteria^ or other organisms. The neck of the flask was 

 then again closed. On the twelfth day the fluid had stiU 

 undergone no change in appearance, and when examined 

 microscopically it still showed no trace of organisms, though 

 the fluid was — as it had been at the time of the first exami- 

 nation — full of minute, undissolved particles of carbolic acid. 



Fluids boiled for five minutes, and half -filling hermetically 



Sealed Flasks containing Ordinary Air. 



F 



No. XLII. Hay Infusion, after forty-eight hours, showed 

 no change, and continued to remain quite clear and free 



^ It had been rendered turbid from the first, by the carbolic acid. 

 ^ The fluid had been rendered paler and turbid from the first, by the 

 addition of the carbolic acid. 



\ 



