Bakewell. — On Inflammatory Action in dead Animal Bodies. 115 



5 p.m. temp. 90° F, The cut extremity of each piece was cloudy and 

 whitish, as viewed through a lens of 1|- inch focus ; vessels looked more 

 marked and prominent than when put in ; outline of pieces not so sharp 

 and well marked as those in the water. 



" Under the microscope, round nucleated cells were seen projecting 

 from the cut surface ; a few of such cells were floating in the nutrient 

 fluid ; several dark-coloured cells round, containing numerous nuclei, were 

 seen. 



"At 5.30, these were obscured by a cloud of white cells with granular 

 contents. 



" 14th, 9 a.m. : The little phials in which the tails had been placed 

 showed a white cloudy precipitate, about J inch deep. On microscopical 

 examination, this proved to be granular protoplasm in amorphous masses, 

 but showing faintly a commencement of segregation into cells. In the fluid 

 were floating about (A) innumerable altered red corpuscles ; (B) many 

 round cells of a yellowish or fawn colour, containing two or three nuclei ; 

 (C) innumerable leucocytes ; (D) columnar and other epithetial cells. 



" The tails themselves showed the following changes: — 1st, all the red 

 corpuscles were gone ; none were to be seen in the most transparent parts ; 

 2nd, all the pigment cells were broken up into small portions, irregular in 

 outline, but much rounder and less angular than the normal pigment cells 

 of the tadpole ; 3rd, the striped muscular tissue was in a state of incipient 

 fatty degeneration ; 4th, over the whole of the skin were crowds of 

 leucocytes covering it, and easily detached." 



This is a fair sample of scores of similar experiments. They proved that 

 leucocytes originated in an inflamed part, and were not brought to it from 

 the blood. 



I then tried what could be done with warm-blooded animals, but was for 

 a long time only partially successful, owing to not using a proper nutrient 

 fluid at a sufficiently high temperature. The following experiment with 

 egg-albumen and water is interesting, as showing that even with this 

 the commencement of inflammation could be produced in a warm-blooded 

 animal. 



December 23rd, 1878. — Placed portions of the liver, the peritonoeum 

 from the mesentery, and voluntary muscle of a duck, in a mixture of egg- 

 albumen and water, kept in contact with my own body. The peritonaeum 

 was in a separate bottle. Between two and three hoiu's afterwards, 

 there was evident (naked eye) turbidity of the albumen in which the peri- 

 tonceum was placed ; the membrane appeared swollen and milky. The 

 turbidity of the bottle in which the muscle and liver were placed was not so 

 marked. Both appeared paler. 



