393 



Penicillium Grown in 10% Tomato Gelatin. 100 cc. 70° F. 



Per Cent 

 Sod. Benz. 



Time to 

 Germ. 

 Hours. 



Development. 









24 



White colonies dotting surface. 



96 hours — surface covered, green, mature. 





1-12 



24 



Same as control. 





1-10 



24 



Same as control. 





1-8 



24 



Same as control. 





1-6 



24 



Less developed than control. 



96 hours — surface covered, nearly all green. 



432 hours — mycelium curled up round edge . 





1-4 



48 



Small white colonies dotting surface . 



96 hours — surface covered, nearly all green. 



342 hours — mycelium curled from edges to center. 





1-2 



72 



Small white colonies dotting surface. 



96 hours — surface about two-thirds covered, center green. 



342 hours — mycelium curled up so as to enclose the spores. 





In this experiment in which a solid medium was used, the effect of 

 the sodium benzoate on the development of the mould was not marked, 

 except in the cultures containing the larger amounts. In these there was 

 a slight retardation, and also a curling up by the mycelium from the sub- 

 stratum. 



Penicillium Grown in Tomato Bouillon in Moist Chambers, 70° F. 



Per Cent 

 Sod. Benz. 



Time to 



Germ. 



Hours. 



Development. 



1-12 



1-10 



1-8 



1-6 

 1-2 



24 



24 



24 



24 



48 

 96 



Short tubes formed. 



48 hours — well developed colonies formed. 



Short tubes formed. 



48 hours — colonies smaller than in the control. 



Tubes just forming. 



48 hours — less development than in the 1— 12th solution. 



Less than in the l-10th solution. 



48 hours — less than in the 1— 10th solution. 



Spores germinated, shorter tubes than in the 1— 8th solution. 



Spores germinated, short tubes. 



Note. — In 120 hours the control was exhausted, having empty hyphse ; the 

 other cultures, with the exception of the h<yh solution, have hyphae with many 

 vacuoles in the protoplasm, the conidiophores formed are apparently normal. 



