394 



The effect of the antiseptic on the development of the mould grown 

 in the moist chambers was not so pronounced as when a larger quantity 

 of solution was used. Neither was the effect always uniform ; sometimes 

 the spores in the |% and the \% solutions merely swelled, but no develop- 

 ment of hyphse occurred; in others short tubes developed from some of 

 the spores, while still other spores showed no changes whatever. 



To test the effect of the larger quantity of solution, inoculations were 

 made into flasks containing 100 and 500 cc, respectively, of the solutions. 

 The results indicated that the effect of the antiseptic on the mould de- 

 velopment was greater when grown in the larger quantity of the solution. 



Penicillium Grown in Tomato Pulp, in Petri Dishes, 65° F. 



Per Cent 

 Sod. Benz. 



Time to 

 Germ. 

 Hours. 



Development. 



1-12 



1-10 



1-8 



1-6 

 1-4 

 1-2 



72 



144 

 192 

 144 



White colonies dotting surface. 



96 hours — spores formed. 



192 hours — surface covered, green. 



White colonies growing up on side. 



192 hours — spores formed on one side, colonies starting in center. 



Colonies started in center. 

 312 hours — spores formed. 



White colonies growing up one side. 

 192 hours — spores formed. 









The pulp used in the experiments was of fine quality, and without 

 any added ingredients such as are used in ketchup, and was used so as to 

 determine the action of the sodium benzoate alone in the pulp. During 

 the early stages of development, the mould grows down into the pulp, so 

 that the whole surface of the hyphse acts as an absorbent and would thus 

 be affected to a greater extent than where only a part of the surface was 

 in contact. This may serve to explain the more pronounced action of the 

 sodium benzoate when in the pulp, and also the fact that after the mould 

 has developed sufficiently to grow out of the pulp the development becomes 

 more nearly normal. 



