396 



had 200 cc. water added, then stood for 12 hours, after which the juice 

 was filtered. 



3. This was similar to 2, but the vessel was covered closely during 

 the steaming. 



For the experiments, 50 cc. of the filtrate from each set were placed 

 in flasks. They were inoculated • with the mould without any previous 

 sterilization. The following table shows the time required for, and the 

 effect on, development : 



Penicillium Grown in Cranberry Juice. 



Medium. 



Days to 

 Germinate. 



Development. 



Raw juice 



4 

 2 

 2 

 3 

 2 

 2 



Short tubes. 



7 days — only small white colonies. 





7 days — colony green. 





7 days — surface green. 





7 days — surface green. 



Juice cooked, closed + lOcc. water 



7 days — surface green. 



Surface nearly covered. 

 7 days — surface green. 



After two weeks' development, the color of the spores of Penicillium 

 was a yellowish green, instead of the normal bluish 'green, and the my- 

 celium was very scantily developed. The surface had a somewhat granu- 

 lar appearance, instead of the smooth, even appearance of a normal culture. 

 The filaments, when seen with the microscope, were thin, shrunken, and 

 clear, with distorted outlines. The cultures were kept for months, re- 

 maining scanty and granular looking, and a peculiar feature was that no 

 development of bacteria occurred, even in the uninoculated ones, though 

 no sterilization had been done, and the uninoculated were exposed to the 



