409 



GERMINATION FROM PRESERVATIVE MATERIAL. 



To determine if there were a permanent deleterious effect produced 

 on the plant through the toxic effect of the chemicals, inoculations were 

 made from two weeks* old cultures into tomato bouillon. The result is 

 shown in the table : 



Germination of Penicillium Grown in Preservative Solutions 



14 Days. 



Preservative. 



Control 



Sodium benz. 1-10% . . . 

 " 1-5%... 

 " 1-2%... 



Benzoic acid, 1-10%. . . 

 " 1-5%... 

 " 1-2%... 



Borax, 1-10% 



" 1-5% 



" 1-2% 



Boric acid, 1-10% 



" 1-5% 



" 1-2% 



Sod. salicylate, 1-10%.. 

 " " 1-5%. . 



" " 1-2%.. 



Salicylic acid, 1-10% . . . 

 " 1-5%... 

 " 1-2%... 



Sod. sulphite, 1-10% . . . 

 1-5%... 

 1-2%... 



Saccharin, 1-10% 



1-5% 



1-2% 



Copper sulphate, 1-10% 

 1-5% 

 1-2% 



Number 



Days to 



Germinate. 



Stage of Development in 5 Day=. 



Surface covered, green. 



Surface covered, green. 



Thin ring, having blue dots. 



Surface nearly covered, green. 

 Small surface colonies, blue . 



Surface covered, green. 

 One surface colony, green. 



Surface covered, green. 



" nearly covered, green in center. 

 Few submerged colonies. 



Surface covered, green. 



Colonies on surface, green. 

 Surface covered, 



Surface covered, green. 



Colonies on surface, green. 

 Surface covered, 



