91 



Tube Media Test I. 



Sample of 6/14, 1915. 

 Sample from Tree XIII— 13JPlot F. 

 Laurel. 



One ce. portions of the 1-400,000 dilution of the sample were plated on 

 the following media: 



Lipman and Brown agar. 



Conn's sodium asparaginate agar. 



Agar alone. 



Soil and agar (Purdue soil). 



Soil extract (autoclaved) and agar. 



Soil extract (unheated) and agar. 



(15 gms. agar in all media.) 



Transfers were made from best colonies on each media to slants of other 

 media. Tables give results of growth on these agar slants at end of 5 and 

 14 clays' incubation at 22° C. 



8 Colonies from L and B agar to 



Xa. asp. agar 



Soil ext. (unheated) . 



Soil ext. (autoclaved). 



Agar alone. 



Agar and soil. 



5 Days. 



14 Days. 



|8 g.* 



8g- 



— 



— 



j6g. 



7 g. 



12- 



1 — 



f5g. 



5 g. 



3 — 



J5g. 



5 g. 



13- 



3 — 



J4g. 



5 g. 



U- 



3 — 



*S = growth. — =no growth unless otherwise specified. 



