94 



25 transfers to Soil oxt. (autoclaved) 20 made grow I b . 



25 transfers to Agar alone 20 made growth. 



25 transfers to Agar and soil 19 made growth. 



Notes. 



When tubes of organisms grown originally on same media were put aide 

 by side the following differences were noted. 



(1) Agar alone supported very poor growths. 



(2) Agar and soil supported fully as poor growths as agar alone. 



(3) The two extracts acted about the same, although the heated extract 

 grew the organisms originally grown on Na. asp. agar a little the best. 



(4) L. and B. agar and Na. asp. agar supported good growths. 



(5) From any macroscopic test the growths on the L. and B. agar were 

 far superior to those on the Na. asp. agar. 



Tube Media Test II. 



Samples of 6/14, 1915. 

 Samples from Tree VI— 24. Plot C. 

 Laurel. 



One cc. portions of the 1-400,000 dilution of the sample were plated on 

 the following media: 



Lipman and Brown agar. 



Conn's sodium asparaginate agar. 



Agar alone. 



Soil and agar (Purdue soil). 



Soil extract (unheated) and agar. 



Soil extract (autoclaved) and agar. 



(15 gms. agar in all media.) 



Transfers were made from best colonies on each media to slants of other 

 media. Tables give results of growth on these agar slants at end of 5 and 

 14 days' incubation at 22° C. 



