96 



A ah . . 



When tubes of organisms grown originally on same media wen pui side 

 by side the following differences were noted: 



(1) Agar alone supported very poor growths. 



(2) Agar and sod supported fully as poor growths as agar alone. 



(3) The two extracts acted about the same, although the heated extract 

 grew the organisms originally grown on Na. asp. agar a little the best. 



(4) L. and B. agar and Na. asp. agar supported good growths. 



(5) From any macroscopic test the growths on the L. and B. agar were 

 far superior fin those on the Xa. asp. agar. 



Tube Media Test ill. 



Samples of 6/25, 1915. 

 Sample No. 6. Rye Plot. 

 Cover Crop Investigations. 



One ce. portions of the 1 to 400,000 dilution of this sample were plated 

 on the following media: 



Lipman and BroAvn agar. 



Conn's sodium asparaginate agar. 



Agar alone. 



Soil and agar (Purdue soil). 



Soil extract (unheated) and agar. 



Soil extract (autoclaved) and agar. 



(15 gms. agar in all media.) 

 Colonies developing well on first two media listed were put on other 

 media and growth noted at end of 5, 11. and 15 days' incubation at 22 I '. 



From .'/ Coloni&& <>n L and li agar to 



Xa. asp. agar 



Soil ext. (unheated) 

 Plain agar 



Days. 



11 Days. 



3g. 



1 — 

 2g. 



4 g- 



2 g. 



2 — 



2 



3g. 



3 g. 



1 — 



1 — 



15 Days. 



4 g. 



3 g. 

 I — 

 3 g. 

 1 — 



