97 



From k Colonics on Xa. asp. agar to 



L and B agar 



Soil ext. (unheated). 



Plain agar 



5 Days. 



ll 



Days. 



15 



Days. 



4g. 





4 g. 





4g. 



(2g. 





3 g. 





4g. 



l2- 





1 — 







:3g. 





3 g. 





3 g. 



ti- 





1 — 





1 — 



Summary 5 Days. 

 4 transfers ro L and B agar 4 made growth. 



4 transfers to Xa. asp. agar 3 made growth. 



8 transfers to Soil ext. (unheated) ~. 4 made growth. 



5 transfers to Agar alone 6 made growth. 



Summary .15 Days. 

 4 transfers to L and B agar 4 made growth. 



4 transfers to Xa. asp. agar 4 made growth. 



8 transfers to Soil ext. (unheated - ) 7 made growth. 



5 transfers to Agar alone 6 made growth. 



Xotes. 



When tubes of different media containing the same organism from the 

 same original colony were put side by side, the following was noted: 



(1) The growth on agar alone, soil and agar or on soil extract (unheated) 

 was small. 



(2) The soil extract carried a better growth than the soil alone. 



(3) L. and B. agar and Xa. asp. agar carried a good growth. 



(4) There was more development of distinguishing characteristics as to 

 form of streaks and chrornogenisis present, with the L and B agar. 



Tube Media Test IV. 



Samples of 6 25, 1915. 

 Sample Xo. 7. Clean Culture Plot. 

 Cover Crop Investigation. 



One ee. portions of the 1 to 400,000 dilution of this sample ^vere plated 

 on the following media: 



Lipman and Brown agar. 

 Conn's sodium asparaginate agar. 



5084 — 7 



