100 



(,'/ rural Xotrs. 



When tubes of differenl media containing the same organism from the 

 same original colony arc pul side by side, the following is noted: 



(1) The growth on agar alone, soil and agar or on soil extract (unheated) 

 is small. 



(2) The soil extract carries a better growth than the soil alone. 



(3) L. and B. agar and Na. asp. agar carry a good growth. 



(4) There is more development of distinguishing characteristics as to 

 form of streaks and chromogcnisis present, with the L. and B. agar. 



Tube Media Test V. 



Sample of 7/1(5, 1915. 

 Sample No. 8. Millet Plot, 

 Cover Crop Investigations. 



One ce. portions of the 1 to 400, 000 dilution of this sample were plated 

 on the following media: 



A. Wheat straw extract. 



B. Leaf extract, 



C. Starch. 



D. Agar alone. 



E. Ammonium nitrate. 



F. Conn's sodium asparaginate. 



G. Soil. 



H. Soil and starch. 

 I. Lipman and Brown agar. 

 J. Ammonium nitrate and starch. 

 (15 gms. agar is basis of all media.) 



Colonies developing well on each meaia, plates 1 1 1 and IV, were transferred 

 to slants of other media. Tables give results of growth on these slants at 

 end of 6, 10 and 14 days' incubation at 22° Centigrade. 



