105 



Summary 6 Days. 



12 transfers to Wheat Straw Ext 9 made growth. 



12 transfers to Leaf Ext 5 made growth. 



32 transfers to Starch 27 made growth. 



12 transfers to Agar alone 10 made growth. 



12 transfers to Ammonium Nitrate 12 made growth. 



32 transfers to Na. asp. agar 28 made growth. 



12 transfers to Soil 10 made growth. 



20 transfers to Soil and Starch 15 made growth. 



32 transfers to L and B agar 30 made growth. 



12 transfers to NH4NO3 and St 10 made growth. 



12 transfers to Soil and NH4NO3 - 9 made growth. 



12 transfers to Soil Ext 12 made growth. 



212 transfers 177 made growth. 



Summary 14 Days. 



12 transfers to Wheat Straw Ext 9 made growth. 



12 transfers to Leaf Ext , . . . . 5 made growth . 



32 transfers to Starch 28 made growth. 



12 transfers to Agar alone 11 made growth. 



12 transfers to Ammonium Nitrate 12 made growth. 



32 transfers to Na. asp. agar . 31 made growth. 



12 transfers to Soil 10 made growth. 



20 transfers to Soil and Starch 15 made growth. 



32 transfers to L and B agar 32 made growth. 



12 transfers to NEUNO3 and St , 11 made growth. 



12 transfers to Soil and NHjNOj 11 made growth. 



12 transfers to Soil Ext 12 made growth. 



212 transfers 187 made growth. 



Notes. 



(1) In this set of tests, as in those run previously, there was very little 

 growth on the agar alone, the soil, and the soil extract slants. Practically 

 all the organisms tested made some growth on these media. 



(2) Ammonium nitrate furnishing nitrogen both in NH 4 and N0„ did 

 not grow better cultures than agar alone. This latter is from observations 

 made after fourteen days' incubation. 



(3) Wheat straw extract grew but little better cultures than the soil 

 extract, while leaf extract was a total failure as a media. 



(4) Starch furnishing sources of energy, and being capable of being 



