129 



As a result, the chloroplasts, chondrisomes, etc., are stained a deep blue, 

 the cytoplasm a light orange or almost colorless, and the cell walls varying 

 intensities of orange. 



The writer has never been able to see the use of the treatment with 

 alizarin, and this part of the process may be omitted. However, Benda's 

 solution of alizarin is made as follows: Make a saturated solution of Kahl- 

 baum's alizarin-sulfo-saurem Natron (mono.) in 70 per cent, alcohol. One 

 cc. of this solution is added to 80 to 100 cc. of water. 

 Benda's crystal violet solution is made as follows: 



Saturated solution crystal violet in 70% alcohol 1 part. 



1% hydrochloric acid in 70% alcohol 1 part. 



Anilin water 2 parts. 



50S4 — 9 



