:;:,! 



all but the bottom collection, which was taken in the manner described 

 below and as illustrated by the fiir 



A sixteen-ounce r« -agent bottle (see Kig. 1) with a ground glass stopper 

 was securely fastened to a block of cement weighing approximately 30 lbs. 

 The stopper was bo tied that it could be partly pulled out. A strong cord 

 was attached to the neck of the bottle to permit raising and lowering the 

 bottle. A second cord was attached to the stopper so that when the empty 

 bottle was at the bottom the stopper could be pulled as far as its fastenings 

 would permit, allowing the bottle to be filled with the bottom ooze. When 

 the bottle was filled the cord attached to the stopper was loosened, thus 

 allowing it to snap back in place and securely close the bottle, and with 

 the cord around the neck the bottle was drawn to the surface. The stopper 

 and neck of the collecting bottle were rinsed off first with alcohol, then 

 distilled water. The contents were then transferred to smaller reagent 

 bottles, corked and sealed with paraffin to insure their being air tight. 



The contents of the collections, especially the bottom collection, were 

 examined microscopically and the plants and animals that seemed alive 

 were listed. As a check, some bottles of the same collection were kept fifteen 

 days in darkness and at approximately the same temperature as the lake 

 bottom. Their contents were then examined and the plants and animals 

 found therein were apparently as active as when first collected. The animals 

 were all seen moving with more or less rapidity, the protozoans quite rapidly, 

 the higher forms not so much so. Their activity increased with exposure 

 to light and air. 



From the total examinations made, the following were found, demen- 

 ti d to be alive and classified. Nine protozoa, one rotifer, one crust; 

 twenty algae and fourteen diatoms. 



C Com and Wei 



Protozoa : 



Dactylasphaerium radiosum Ehr. 



Difflugia globostoma Leidy. 



Amoeba proteus Ehr. 

 Helizoa: 



Actinosphaerium eichornii. 

 Mastigophora-flagellata: 



Peranema sp.l 



