42 



Martin J. Prucha 



organism was propagated on media 334, 335, and 337 (page 11), to which 

 were added various amounts of hydrochloric acid (HCl) aiid sodium 

 hydroxide (XaOH). Cane sugar was added in various amounts to 

 medium 334. 



Ten cubic centimeters of the media were introduced into each test 

 tube and sterilized. While the agar was still melted the various additions 

 were made to the tubes, and, after thorough mixmg, the tubes were 

 sloped. 



All cultures were made in duplicate. At the end of three weeks staLi 

 preparations were made from each slope for morphological study, carbol 

 fuchsin being used for staining. The media employed and the results 

 of the morphological study are given in table 7: 



TABLE 7. Morphological Variation of B. radicicola on the Different Media 



Medium 



]Morphological appearance 



Alultiplication 



M-dium 331, 10 cc. 



+ 







+ 







+ 







+ 



1 



+ 



1 



+ 



2 



+ 



3 



+ 







+ 







+ 



1 



+ 



1 



+ 



2 



+ 



3 



+ 







+ 







+ 



1 



+ 



1 



+ 



2 



+ 



3 



+ 



4 



+ 



5 



+ 



6 



+ 



8 



+ 



10 



.1 cc. N/1 HCl 

 occ. N/1 HCl 

 Occ. N/1 HCl 

 occ. N/1 HCl 

 Occ. N/1 HCl 

 Occ. N/1 HCl 

 1 cc. N/1 NaOH 

 5 cc. N/1 NaOH 

 Occ. N/1 NaOH 

 5 cc. N/1 NaOH 

 cc. N/1 NaOH 



cc. N/1 NaOH 



1 per cent cane sugar 

 5 per cent cane sugar 

 per cent cane sugar 

 5 per cent cane sugar 

 per cent cane sugar 

 per cent cane sugar 

 per cent cane sugar 

 per cent cane sugar 

 per cent cane sugar 

 per cent cane sugar 

 per cent cane sugar. 



Small, short cells 



Short rods, slightly stained 



Short rods, slightly stained 



Short rods, slightly stained 



Short rods, slightly stained 



Short rods, slightly stained 



Short rods, slightly stained 



Short rods, well stained 



Short rods, well stained 



Short rods, well stained 



Short rods, well stained 



Short rods, well stained, few small bacteroids 

 Short rods, well stained, few small bacteroids 



Cells vary in size, bacteroids present 



Cells vary in size, bacteroids present 



Cells vary in size, bacteroids .present 



Cells vary in size, bacteroids present 



Cells vary in size, bacteroids present 



Ceils vary in size, bacteroids present 



Ceils A'ary in size, bacteroids more ab'^ndant 

 Cells vary in size, bacteroids more abundant 

 Cells vary in size, bacteroids more abundant 

 Cells vary in size, bacteroids verj^ abundant . 

 Cells vary in size, bacteroids very abundant 



Fair 



Fair 



Poor 



None 



None 



None 



None 



Good 



Good 



Poor 



Poor 



Verv poor 



Doubtful 



Good 



Good 



Good 



Good 



Good 



Good 



Good 



Good 



Fair 



Fair 



Poor 



