Studies of Bacillus Radicicola of Canada Field Pea 43 



TABLE 7 (concluded) 



Medium 335, 10 cc. 



+ 



+ 0.1 cc. N/1 HCl. . 

 + 0.5CC. N/1 HCL. 

 4- 1.0 CC. N/1 HCl.. 

 + 1.5 cc. N/1 HCL. 

 -{-2.0cc. N/1 HCL. 

 + 3.0cc. N/1 HCL. 

 + 0.1 cc. N/1 NaOH. 

 + 0.5CC. N/1 NaOH. 

 + l.Occ. N/1 NaOH. 

 + 1.5cc. N/1 NaOH 

 + 2.0cc. N/1 NaOH 

 + 3.0 cc. N/1 NaOH 



Medium 337, 10 cc. 



+ 



+ 0.1 cc. N/1 HCL . 

 + 0.5CC. N/1 HCL. 

 + l.Occ. N/1 HCL . 

 + 1.5 cc. N/1 HCL . 

 + 2.0cc. N/1 HCL. 

 + 3.0cc. N/1 HCl. . 

 + 0.1 cc. N/1 NaOH 

 + 0.5CC. N/1 NaOH, 

 + l.Occ. N/1 NaOH 



+ 1.5cc. N/1 NaOH, 



+ 2.0CC. N/1 NaOH 



+ 3.0cc. N/1 NaOH 



Variation in size and shape, few bacteroids. . 

 Variation in size and shape, few bacteroids. . 



Very few rods 



No organiMns visible 



No organisms visible 



No organisms visible 



No organisms visible 



Variation in size and shape, few bacteroids. . 

 Variation in size and shape, few bacteroids. . 

 Greater variation, more bacteroids than in . 5 

 Greater variation, more bacteroids than in . 5 

 Greater variation, more bacteroids than in . 5 

 Cells stained deeper than others, bacteroids 

 present 



Good 

 Good 

 Poor 

 Doubtful 



None 

 None 

 None 

 Very good 

 Very good 

 Good 

 Good 

 Poor 



Doubtful 



Pronounced variation in shape and size of cells. 



Small cells, irregular shape and size 



Very slender cells, irregular shape and size. . . . 



Extremely small cells 



Extremely small cells 



Extremely small cells 



No organisms visible 



Short; irregular cells 



Short, irregular cells 



Shape and size extremely varied, many bac- 

 teroids 



Large cells, varying in shape and size, many 

 bacteroids 



Large cells, varying in shape and size, many 

 bacteroids 



Very large cells, varying in shape and size, 

 many bacteroids 



Good 



Good 



Poor 



Very poor 



Doubtful 



None 



None 



Abundant 



Abundant 



Abundant 



Good 



Good 



Poor 



In order to test the infecting power of the different cultures, three 

 flowerpots of alfalfa plants grown in sterile soil were inoculated with 

 the organism from each test tube. Four weeks after inoculation the 

 plants were examined for nodule development. The results of the in- 

 oculations are presented in table 8. Fifty-eight plants in flowerpots 

 were used as controls. These plants were grown in sterile soil and were 

 not inoculated. The control plants were distributed among the other 



