Studies of Bacillus Radicicola of Canada Field Pea 45 



TABLE 8 (conchided) 



Medium 



Number 



of 



plants 



inoculated 



Total 

 number 



of 

 nodules 



Number 



of 

 nodules 

 per plant 



Medium 337, 10 cc. 



+ 



+ 0.1 cc. N/lHCl... 

 + 0.5 cc. N/1 HCl... 

 + 1.0 cc. N/1 HCl... 

 + 1.5 cc. N/1 HCl... 

 + 2.0cc. N/1 HCl... 

 + 3.0 cc. N/1 HCL. . 

 + 0.1 cc. N/1 NaOH 

 + 0.5 cc. N/1 NaOH 

 + 1.0 cc. N/1 NaOH 

 + 1.5cc. N/1 NaOH 

 + 2.0cc. N/1 NaOH 

 + 3.0cc. N/1 NaOH 



Controls 



23 

 32 

 37 

 36 

 36 

 33 

 32 

 26 

 27 

 39 

 31 

 32 

 33 



54 



50 



110 



77 



23 



94 



132 



80 



120 



174 



114 



154 



87 



2.3 

 1.6 

 3.0 

 2.1 

 0.6 

 2.8 

 4.L 

 3.1 

 4.4 

 4.5 

 3.7 

 4.8 

 2.6 



735 



•=624 



0.8 



* Nodules were present in only thirteen out of fifty-eight pots 



. Results 

 The extent of multiplication and the description of the morphological 

 characters of the organisms in the various media are summarized in table 7. 

 No visible increase in the number of organisms was found when 1 cubic 

 centimeter or more of the normal solution of HCl was added to 10 cubic 

 centimeters of each medium. The few organisms found on these slopes 

 were probably those that were introduced when the slopes were inoculated. 

 In medium 337 slight multiplication took place on slopes of 10 cubic 

 centimeters of the medium plus 1 cubic centimeter of the normal solution 

 of HCl. The best multiplication occurred in media 335 and 337 when 

 0.1 to 1 cubic centimeter of the normal solution of NaOH was added 

 to 10 cubic centimeters of the medium. This was particularly noticeable 

 in medium 337. The addition of 3 cubic centimeters of the normal solu- 

 tion of NaOH to 10 cubic centim_eters of the medium practically inhibited 

 multiplication. The bacteroids developed more readily when NaOH was 

 added. 



