50 



Martin J. Prucha 



TABLE 10 (concluded) 



Number of organisms on one agar slope 



Medium 334 with 



Cultures 

 three weeks old 



Cultures 

 ten weeks old 



Cultures 



twenty 



weeks old 



2 per cent cane sugar No count made 



10.0 per cent cane sugar No count made 



20 . per cent cane sugar No count made 



40 . per cent cane sugar ; No count made 



2 per cent dextrose No count made 



2 . per cent levulose No count made 



2.0 per cent lactose : No count made 



2 . per cent galactose No count made 



2.0 per cent glycerin , No count made 



2 . per cent mannite No count made 



. 1 per cent asparagin i No count made 



1 . per cent salicin No count made 



. 5 per cent amygdalin I No count made 



. 5 per cent resorcin j No count made 



.0.2 per cent phloroglucin | No count made 



. 1 per cent potassium nitrate ' No count made 



0.2 per cent potassium nitrate i No count made 



. 6 per cent potassium nitrate I No count made 



0.1 per cent calcium nitrate j No count made 



0.2 per cent calcium nitrate | No count made 



. 6 per cent caicium nitrate ! No count made 



1 . per cent gelatin : No count made 



5 . per cent gelatin ' No count made 



1 . per cent Witte's peptone No count made 



2.0 per cent Witte's peptone ' No count made 



5.0 per cent Witte's peptone ; No count made 



0.2 per cent Alerck's peptone | No count made 



1.0 per cent Merck's peptone | No count made 



3.0 per cent Alerck's peptone I No count made 



. 5 per cent potassium oxalate j No count made 



. 5 per cent potassium citrate No count made 



300,000,000 



3,000,000 



1,000,000 



16,000,000 



31,000,000 



400,000 



Plates spoiled 



3,000,000 



250,000,000 



300^000^000 



No colonies 



250,000,000 



No colonies 



Few 



120,000,000 



74,000,000 



259,000,000 



280,000,000 



150,000,000 



400,000,000 



150,000,000 



50,000,000 



50,000 



Contaminated 



No colonies 



22,000,000 



300,000,000 



No colonies 



No colonies 



No colonies 



90,000,000 



5,000,000 



10,000 



No colonies 



52,000,000 



No colonies 



180,000 



600,000 



20,000,000 



25,000,000 



108,000,000 



No colonies 



No colonies 



No colonies 



No colonies 



72,000,000 



224,000,000 



144,000,000 



486,000,000 



108,000,000 



130,000,000 



64,000,000 



Plates spoiled 



216,000,000 



648,000,000 



No colonies 



8,000,000 



324,000,000 



No colonies 



No colonies 



No colonies 



of B. radicicola on the agar slopes to which the various chemicals were 

 added. The number of organisms in the cultures on the various media 

 at the tune they were tested for their efficiency to produce nodules is given 

 in table 10. The results of the inoculations are sho^m in tables 11, 12, 

 and 13. 



Results 



Growth and number of organisms (tables 9 and 10) 



Bacillus radicicola of Canada field pea produces scant growth on agar 



