402 F. M. Rolfs 



containing twenty cubic centimeters of the medium developed single 

 surface colonies in thirty days which measured from sixteen to thirty 

 millimeters in diameter. The older colonies were amber yellow (4) in 

 color, were convex, showed a dark central nucleus, and were often con- 

 centrically ringed. The smaller, thin colonies had a semi-transparent 

 luster. 



Hiss-glucose streaks. — On Hiss-glucose slant-tube medium (+15), the 

 streak having been made with a millimeter loop, growth was noticed in 

 forty-eight hours at room temperature of about 25° C. A convex, slimy, 

 straw yellow (2) growth developed along the path of the loop. The streak 

 widened gradually (chiefly toward the bottom), soon became filiform, and 

 in ten days completely covered the slant with a slimy yellow (2) growth. 

 In culture three or four months old the medium was browned. Some 

 of the growth soon moved downward and formed a slimy deposit at the 

 base of the slant, which finally became amber yellow (4) in color. As the 

 medium dried out the slimy deposit gradually settled, and in the five- 

 and six-months-old cultures there was a slimy, amber yellow (4) bacterial 

 deposit from five to ten millimeters deep in the bottom of the tube. 

 Inoculated tubes placed in the incubator at about 37° C. also covered the 

 slants in ten days, but the growth was of about the same color as the 

 medium. 



Hiss-glucose stab. — In Hiss-glucose stab the growth was filiform and 

 best toward the top of the stab, there being very little or no growth in 

 the lower part of the tube. A circular growth formed on the surface at 

 the point of inoculation, and covered the surface in six or seven days. 

 The medium was not liquefied nor softened. 



Potato agar streaks. — In potato agar a slightly raised, wet, shining 

 growth developed along the path of the needle in two or three days at 

 room temperature of 25° C. The streak gradually widened, and finally 

 covered about two-thirds of the surface of the slant, becoming amber 

 yellow (1) in color. Some of the shmy growth soon moved downward 

 and formed a deposit at the base of the slant. Growth was moderate, 

 never completely covering the slant. 



Dextrose litmus agar streak. — In dextrose litmus agar, there was slight 

 growth along the path of the needle in two days. The streak was slightly 

 elevated, and widened most rapidly at the bottom, becoming filiform. 

 The slimy growth passed downward and accumulated at the base of the 



