404 F. M. Rolfs 



inoculated with a one-millimeter loop of beef broth culture three days 

 old, and kept at 25° C. Two days after inoculation there was a slight 

 whey ring at the surface of the milk; after four days the ring was three 

 or four millimeters deep. Smith (1903) states that this organism slowly 

 precipitates casein, which is then gradually redissolved. 



In about ten days the whey ring was five or six millimeters deep and 

 was amber yellow (3) in color, and the fluid curd gradually changed to 

 yellowish tan (4) in color. The amount of clear whey gradually increased, 

 and in about twenty-five days it became gamboge yellow (2) and the 

 curd gradually became sienna (4) in color. The whey was viscid, while 

 the mobile precipitate finally became a compact, slimy mass. An amber 

 yellow pellicle was formed in from fifteen to twenty days. Filamentary 

 threads often extended from the pellicle to the curd below. The pellicle 

 finally settled. On shaking tubes containing a well-formed pellicle, yellow, 

 slimy threads sometimes extended from the surface of the medium to the 

 bottom of the tube. 



An amber yellow (2) layer of the organism soon formed on the curd, 

 and gradually lowered in the tube as the curd dissolved. About the 

 twenty-second day many white tyrosine crystals formed in this layer. 



About the forty-fifth day the pellicle in many cases had settled. The 

 whey now constituted three-fourths of the material, was viscid, and was 

 saffron yellow (4) in color. The precipitate was a yellow-buff (1), slimy 

 mass dotted with white tyrosine crystals. 



In four-months-old cultures the material was about one-half dried 

 out and the whey constituted about one-half of the material. It was 

 Indian chestnut-red (2) in color, with a thick, slimy, fawn-colored (2) 

 coagulum dotted with white tyrosine crystals. 



Litmus milk. — In the test with litmus milk, the culture material had 

 been incubated at 37° C. for forty-eight hours just before the inocu- 

 lations were made. It was violet (4) in color, and a one-millimeter 

 loop of a three-days-old beef broth culture was used for inoculating 

 each tube. 



On the second day a bluish violet (4) ring two millimeters deep had 

 formed on the surface of the medium, and by the fourth day the surface 

 ring was from six to eight millimeters deep, in many cases having an 

 amber yellow (1) rim. The material below the bluish violet (4) ring 

 still retained its original color, but gradually became dark violet. 



