A Bacterial Disease of Stone Fruits 405 



In about fifteen days the top ring became pansy violet (4) in color 

 and was from eight to ten millimeters wide. The amber yellow (4) rim 

 was more prominent and usualty a pellicle was formed. In some cases 

 the deposit below the whey had changed to madder brown (2) in color. 



By the twenty-fifth day an amber yellow (1) pellicle had formed, in 

 some cases with filamentary threads extending to the deposit below. On 

 shaking of the tubes, the pellicle formed into thick, slimy threads some- 

 times extending from the surface of the medimn to the bottom of the 

 tube. In some cases the litmus was wholly reduced by the twenty-fifth 

 day. The whey ring was about twenty-five millimeters deep, with the 

 upper five millimeters pansy violet (3) and the lower twenty millimeters 

 yellow lake (3) in color. The deposit in the bottom of the tube was 

 brownish drab (2) in color, and was covered with an amber yellow (1) layer 

 of the organism often three to four millimeters deep. Many white tyrosine 

 crystals had formed in this layer. In about forty-five daj^s the pellicle 

 had settled, leaving a dull yellow-green (1) rim. The litmus had been 

 in some cases completely, and in others only partially, reduced, but the 

 material in most of the tubes had returned to a darker color. The whey 

 by transmitted light was plum-violet (4) in color. There was a thick, 

 slimy, brown-drab (2) deposit in the bottom of the tube, about 10 milli- 

 meters deep, and on the top of this an amber yellow (2) layer of the organ- 

 ism. Tyrosine crystals were scattered throughout the entire slimy mass. 



In four-months-old cultures one-half of the material had dried out. 

 The peUicle had settled, and a plum-violet (3) whey ring about twenty 

 millimeters deep covered a thick, slimy, brownish drab (2) deposit six 

 millimeters deep in the bottom of the tube. An amber yellow (2) layer of 

 the organism on the top of the deposit was still rather prominent. Masses 

 of white tyrosine crystals appeared throughout the entire slimy deposit. 



Fermentation tubes. — A basal solution was made by adding two per cent 

 of Witte's peptone to water. Six solutions were made from this, each 

 containing one per cent, respectively, of one of the following carbon 

 compounds: glycerin, saccharose, mannite, dextrose, maltose, lactose. 

 Five fermentation tubes were filled with each of these solutions and 

 sterilized by heating for twenty-five minutes on three successive days. 

 Three tubes of each set were inoculated and two were left as a control. 

 The inoculated tubes each received a one-milhmeter loop of culture 

 material from a five-days-old peach bouillon culture. 



