82 R. A. EMERSON 
Dilute Dilute 
purple canned Brown Green Total 
Anther color Purple Green Pink Green Purple Pink Green Green 
Ta Ig Ila Ilg Illa IVa V_ Illg, [Vg, VI 
Plant color Purple Purple Sun red Sun red 
Colored aleurone: 
Observed...... 48 25 11 12 16 4 0 10 126 
Calculated... .. 47 24 16 8 16 5 0 10 126 
Difference..... +1 +1 —5 +4 0 —1 0 0 0 
Colorless aleurone: 
Observed...... 78 11 23 4 23 6 42 40 227 
Calculated... .. 62 10 21 4 21 7 55 47 227 
Difference..... +16 +1 +2 0 +2 —l1 —13 —7 0 
In view of the rather large number of plant-color classes and the com- 
paratively small number of individuals concerned here, the fit of the 
observed to the theoretical distribution is remarkably good. The devia- 
tions are such as might be expected by chance seven times in ten trials 
for the colored-seeded lot (P = 0.70), and about once in four trials for the 
colorless-seeded lot (P= 0.26). In addition to this comparison of the 
lot as a whole, it.should be noted that, while among the purple and sun red 
plants as a whole the expected relation of colored (purple and pink) 
anthers to colorless (green) anthers is 3:1, for the colored-seeded lot it 
is 2:1 and for the colorless-seeded lot it is 6:1. The observed relations 
were 59:37 and 101:15, or about 1.6:1 and 6.7:1, respectively. On the 
whole, therefore, this comparison, involving aleurone color as well as plant 
color, supports the suggested factorial interpretation. 
Later behavior of F2 purple I— Only three F: purples with purple anthers 
were tested in F;. One of these, 2960-9, resulted in purple plants with 
purple, Ia, and green, Ig, anthers, and sun red plants with pink, Ila, and 
green, [Ig, anthers, in the respective numbers 14:9:6:3. <A purple plant 
of the genotype 4 ABB Pl plk’r’ should give these four classes in 
the relation 18:6:6:2. The observed deviations might be expected twice 
in five trials, P equaling 0.41. 
Another F2 purple plant, 2958-8, gave F; progeny consisting of the same 
eight color types as were seen in F2 in table 36 (page 146). Evidently the 
F, purple plant was Aa BbPIpl R’ 7’. The deviations from expecta- 
tion are such as might occur by chance in about seventeen out of any 
twenty such trials, P equaling 0.86. The comparison follows: 
