Puant Coiors IN MaIze 91 
nly one plant of each of the missing classes was to have been expected, 
and the distribution as a whole was not far from expectation, P equaling 
0.59. Both the types lacking in F; occurred in Fy, a pink-anthered sun 
red F; producing sun reds and dilute sun reds, while green-anthered 
purples produced in one instance purples, sun reds, and greens, and in 
another instance purples and greens only, all with green anthers. This 
F; lot may consequently be regarded as A A Bb Pl pl R’7’, and therefore 
equivalent to the F2 lot from which it came, and its Fs progenies equivalent 
to F3 progenies. 
A second F,2 purple-anthered purple was backcrossed to green plants 
of types [Vg and VIc (group 1, table 39). From the backcross with green 
of type IVg, A b pl R’, five major color types appeared and both the 
purple and the sun red types contained subtypes with colored and with 
green anthers. While all the classes expected from an F»2 of the genotype 
AABbPlplkR’r’ occurred, the frequency distribution was so far 
from expectation that there is only one chance in five hundred that the 
observed deviations may have been due to errors of random sampling, P 
equaling 0.002. The expected and observed distributions are as follows: 
Color types Purple Sun red sans ae Green Total 
lato iia ome la Va) blo aig. 
MPSeLVeEd=s ees se. U5 15 5 1 9 45 
alculateds ee... ee. 9 9 9 9 9 45 
Whether the discrepancy is genetically significant or was due to some acci- 
dent of pollination cannot now be determined. A backcross of the same F»2 
plant with green of type VIc, ab plr’, yielded only four color types, as 
expected (group 1, table 39), the anthers being colored in all cases. The 
excess of purples and deficiency in two other classes makes the deviations 
from expectation fairly great, so that there is only about one chance in 
seven that they may have been due to errors of random sampling, P 
equaling 0.14. The comparison follows: 
