96 R. A. Emerson 
AaBBPIPIR‘%r’. An F2 progeny was grown from only one F, plant, 
6653-6, resulting in two major color types, purple and brown, in approxi- 
mately a 3:1 ratio. The purples were, as expected, of two subtypes, 
one with purple and the other with green anthers. The theoretical rela- 
tion of 9:3:4 was realized so closely that the observed deviations might 
be expected by chance in at least two out of three trials, z* equaling 0.76 
(when z2=1 and n’=3, P=0.61). The comparison follows: 
Purple, Purple, 
Colors hypess purple anthers green anthers =aonas stot 
la Ig V 
Observed? 0222 238 23 5 9 37 
Calculated ae"2 see 21 2 9 37 
Ditterence 4 centers +2 — 0 | 0 
A second F, plant, 6653-2, was backcrossed with green IVg, A b pl R’, 
resulting in 39 purple plants, 21 with purple and 18 with green anthers, 
where equal numbers were expected, the deviation from expectation being } 
1.5+2.1. The same F,; plant was crossed with a heterozygous dilute } 
sun red, Aabbplpl7r’7’, resulting in 45 purple-anthered purples and 
18 browns, the deviation from the expected 3:1 ratio being 2.25 + 2.32. 
Purple Ig x dilute purple IlIa.— Crosses of green-anthered purple, 
AB PIR’, with dilute purple, Ab Plr’, gave in F; purple-anthered } 
purple, AA Bb PI PIR’r’. The F, should consist of purple-anthered } 
and green-anthered purples, dilute purples, and greens, the three major 
color types appearing in the relation 12:3:1. In F, from a single Fy 
plant, 5263-8, both purple-anthered and green-anthered purples were 
noted, but detailed counts based on anther color were not made. The 
deviations from the expected numbers for the three major types were 
such as might occur by chance in nine out of twenty such trials, P equaling 
0.45. The comparison follows: 
Dilute 
Color types Purple sails Green Total 
Ta, g Illa Illg 
Observed :4. eee sr. sree 36 11 5 52 | 
Calculated 22. ae andeee ce 39 10 3 52 
Differences<¢ pha ee —3 +1 +2 0} 
