106 R. A. Emerson z 
Not only were the frequencies of the major color types fairly close to 
expectation, as indicated above, but the expected subclasses of sun red with | 
pink anthers and with green anthers were observed. Counts of anther. 
color were made in the case of only 65 individuals. These plants were 
distributed to the four color classes, pink-anthered sun red, green-anthered 
sun red, dilute sun red, and green, in the order 24:9:10:22. The 
theoretical distribution of 64 individuals being 27:9:9:19, the deviations 
are such as might occur by chance perhaps twice in three trials, x? equaling | 
0:91 (when: x2 =1-and n’=3)P'= 0:61): 
Only three F. sun reds were tested in F;. One of them (group 2, table 48) ‘ 
bred true sun red, but segregated with respect to anther color. It was there- | 
fore presumably A A BB pl plr’ R’. Two other F2 sun reds (group 3) | 
gave sun red and green offspring in the ratio 229:71, a deviation of only | 
4.0 + 5.1 from a 3:1 ratio. One of these two F, plants was crossed 
with a dilute sun red, resulting in 55 sun red plants. The two F, plants, | 
therefore, were presumably AaB B pl pl. Anther color was not deter- | 
mined, but the fact that the green plants of F; all came from colorless | 
seeds is conclusive evidence for the presence of A a and against the pres- © 
t 
ence of r’ R’. The genotype of the Fy. plants is accordingly set down as 
AaBB pl plr' rr’. 
Green III g x green VIc.— Green plants known to be of type VIe, a 6 pl 7’, 
were crossed with greens which were known to be R’ R% and which from | 
their parentage might have had Pl. The result in F; was dilute purple, - 
supposedly Aabb Pl plr’ R’. Two F-» lots (table 49, group 1) consisted 
of dilute purples, dilute sun reds, and greens in the relation 109:37:135. 
From the assumed genotype of F;, there should occur in F2 the observed 
color types in the relation 27:9:28. The observed frequencies deviated 
from the theoretical ones by amounts such as might occur by chance | 
once in three trials. P equaling 0.33. The comparison follows: 
Color types Se ae Green Total — 
Illa IVa ile TVesWaloge 
Observed 222.038 ce W 109 37 135 281 
Calculated.7. 4a sa 119 40) 123 282 
Difference... Goce —10 —3 +12 —1] 
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