714 Laura FLORENCE 
TECHNIQUE 
In the laboratory the following methods of keeping lice have been tried: 
Opportunities 
ae aay Length 
Conditions Temperature of odin of life 
On laboratory table in petri Room 4 7-10 days 
dishes temperature 
In incubator in open vials 36-34 Ou 4 Under 24 
dry heat hours 
In incubator in vials having in 36°-37° C., 4 3 days 
the bottom a layer of moist moist heat 
cotton batting 4-3 inch thick 
In a gauze bag worn on the body 35°C.+ Constant Lice would 
not feed 
A through 
gauze 
In vials plugged with cotton and 35° C.+& 3-4 Up to 35 days 
gauze and carried close to the 
body day and night 
As in last experiment 30, C.=:: First day. 33 2oidays 
second to 
fifteenth 
day, 2; 
sixteenth 
to twenty- 
sixth day, 1 
We have found the last method the best for rearing hog lice in captivity. 
In the glass containers, hog bristles and teased gauze were provided as a 
foothold. 
Chloroform has been found the most satisfactory medium for killing 
lice both for dissection zn toto and for sectioning. For the former purpose, 
the lice may be preserved indefinitely in 80- to 85-per-cent alcohol, but 
the following fluid has been found much more satisfactory: 
DistilledBwateriak fio. iat Se ek ee eee 30 parts 
AlcoholMOG- pets. cenit isc cc eee 15 parts 
Hormaldehy desta een cee eee 6 parts 
Glacialaceticracid. 2) =.5. 4h re ae 4 parts 
