430 WALLACE LARKIN CHANDLER 
and were fixed on slides by the usual methods. They were then cleared 
and carried down thru the various grades of alcohol to water, and then 
stained for ten minutes in hot (70° C.) 10-per-cent methylene blue in 
saturated anilin oil water (Rasmussen and Myers, 1916). When taken 
from the staining fluid they were hurriedly rinsed in a large volume of 
water, and were then placed directly in 95-per-cent alcohol where they 
were allowed to remain until they were sufficiently destained. They were 
then dehydrated, cleared in xylene, and mounted in balsam. Corre- 
sponding tissues from each of the poisoned animals and from the controls 
were carried thru the same fluids and stained on the same slides. 
DESCRIPTIONS OF EXPERIMENTS 
A large number of experiments were carried out. Since, however, 
the symptoms for each group of animals were in general fairly similar, 
so far as localizing the action is concerned, only two or three experiments 
from each group in which the animals showed typical symptoms are 
included in the descriptions. The other experiments included are those 
in which the symrtoms were different in essential details. 
DOG I (MALE DACHSHUND) 
Weight of dog, 16.4 kilograms. 
August 30, 1916 — Dog fumigated at 26° C. for a period of five and one- 
half hours.? 
Time when fumigation was begun, 11.15 a. m. 
Time when fumigation was finished, 4.45 p. m. 
Observations: After having become accustomed to the strangeness 
of the fumigation chamber, the animal lay down and became quiet. 
At 2 p. m. it was observed that the dog had vomited, urimated, and 
defecated. At 3 p. m. the animal was seen to stagger when attempting 
to walk from one end of the tank to the other. At 4 p. m. the animal 
was found lying on its side; it was unable to lift its head; its respiration 
was labored. At 4.30 the condition was about the same. At 4.45 the 
animal was removed from the tank. The respiration was slow and regular, 
except for intermittent long, deep inhalations; the animal was unconscious; 
9 The chamber was thoroly dehydrated before the experiment was begun, but in this instance no time 
was allowed for saturation. Ordinarily sufficient time was given to insure saturation of the chamber 
before the animal was introduced. 
