444 WALLACE LARKIN CHANDLER 
May 23 — The animal was found with its body raised on its fore legs, 
swaying from side to side, apparently making efforts to stand up but 
its hind legs were useless. The animal had not defecated since being 
placed in the cage, altho it was taken out several times for this purpose. 
May 24 — The animal was very much improved. [t could use its fore 
legs very well and had some use of its hind legs, but when it attempted 
to walk it staggered and fell, or rather tumbled to the floor, striking 
its jaws against the floor with considerable force. It appeared very 
nervous, and was always moving and fidgeting about, apparently 
unable to remain quiet at all. The animal defecated for the first time 
since the beginning of the experiment. 
May 26 — The general condition of the animal was about the same. It 
was taken out on the lawn for exercise. In standing, its hind legs were 
spread far apart. It was unable to walk or to run, but it actually 
tumbled along, jumping high into the air and coming down on its head 
or its back, turning somersaults, or tumbling over sidewise. This dog 
was by nature playful and it had lost none of its playfulness as the result 
of the fumigation; its efforts to play always resulted in its throwing 
itself violently about. An interesting observation was the attempt of 
the dog to go toward any one when called; it made better progress in 
attempting to go in the opposite direction. It apparently was con- 
fused as to distances, and was wholly unable to make progress in a 
straight line.!° 
RABBITS I AND II, AND GUINEA PIGS I AND II 
November 2, 1916 — Animals fumigated at 22° C. for a period of nine 
hours. 
Time when fumigation was begun, 10.30 a. m. 
Time when fumigation was finished, 7.30 p. m. 
Observations: These animals were apparently normal when removed 
from the tank, and never developed any symptoms afterward. 
10 This animal was killed in February, 1918, and a histological examination of the cerebellum revealed 
a striking absence of Purkinje cells. Only from 5 to 10 per cent of the number found in a normal dog 
were present. The contour of the cerebellum was apparently normal, and those Purkinje cells which 
were present were scatterel fairly uniformly thruout the cerebellum. The condition of the animal had 
never improved very markedly; and while it had learned many “ew tricks regarding locomotion, its 
actions were always typically those of a cerebellar animal. 
