PxHyYSIOLoGIcAL ACTION OF NITROBENZENE VAPOR ON ANIMALS 445 
November 18, 1916 — Animals fumigated a second time, this time at 
24° C. for a period of nine hours and thirty minutes. 
Time when fumigation was begun, 10 a. m. 
Time when fumigation was finished, 7.30 p. m. 
Observations: As before, these animals showed no effects of the drug, 
either during the fumigation or afterward. 
RABBIT III AND GUINEA PIG III 
Dec. 22, 1916 — 'These animals were placed together in the fumigation 
chamber at 5.15 p. m., and were allowed to remain there until 4 p. m. 
on December 23. The temperature of the chamber remained constant 
at 20° C. during the first seven hours, then it gradually dropped until 
at the end of the next seven hours it was 15° C., and then it rapidly 
rose to 20° C. again. During the second seven hours no air was intro- 
duced into the tank. 
Observations: The animals nestled together and remained quiet dur- 
ing the entire experiment. A string was tied to the rabbit’s leg, and every 
hour or so the animal’s reflexes were tested. They remained good. Both 
animals were a trifle stupid when they were removed from the tank. 
They were offered food and water, but would not drink and barely nibbled 
at the food. Suddenly the guinea pig fell on its right side and was unable to 
regain its feet. When placed on its left side, it immediately turned again 
to the right side. Violent tremors were observed in all its muscles, and 
presently it was seized with convulsions; all the legs were rigid and the head 
was drawn back on the body. This spasm lasted but a few seconds, and 
when it ended the animal shook itself violently and then executed running 
movements similar to those described in dogs. These movements were 
extremely rapid and lasted until another convulsion appeared. At 8 p. m. 
the guinea pig seemed to be recovering and was able to raise itself on its 
fore legs. It remained quiet for some time, and when observed the next 
morning it was dead. A post-mortem examination showed the lungs to 
be distended and the air passages were filled with blood; the blood was 
dark brown; the liver was congested; the other organs were normal. 
At the end of two hours the rabbit had developed no symptoms, and at 
about 6 p. m. it was again placed in the fumigation chamber. The anima! 
reacted to the jerk of the string until about 9 p.m. It was then removed 
