PHYSIOLOGICAL ACTION OF NITROBENZENE VAPOR ON ANIMALS 449 
April 1 —The condition was about the same, altho the bird appeared 
stronger. When placed on its feet it attempted to walk, but the legs 
stiffened and the bird was thrown forward. 
This bird never fully recovered. It was able to squat on its feet after 
a time, but refused to walk; when urged, it took two or three rapid steps 
and then tumbled over forward. It was unable to remain on a perch, 
falling either forward or backward. The wing movements were well 
coordinated. For a long time the bird was unable to eat without assist- 
ance, but it was finally taught to do so. The bird was killed on June 11, 
in order to make an examination of the brain tissues. 
HEN VI 
March 22, 1917 — Hen fumigated at 27—28° C. for a period of eleven hours. 
Time when fumigation was begun, 10 a. m. 
Time when fumigation was finished, 9 p. m. 
Observations: The bird was removed from the tank apparently 
unharmed. The feces were as described for Hen V. 
March 23 — The bird was apparently well, but not very active. It ate 
eracked corn and drank water. The neck feathers were ruffled. 
March 24 — When first seen on this day (at 8 a. m.) the bird appeared 
normal. It was taken from the cage and placed on the floor. It was 
able to walk and run very well, but suddenly showed a tendency to give 
way to the left side; it flew to a perch 18 inches high, but was unable 
to retain its position; it fatigued very easily. At 10 a. m. the attendant 
reported that the bird had been in convulsions; it appeared normal 
except that it was a little dull. At 3 p.m. the bird appeared to be drowsy; 
it was removed from the cage and placed on its feet; it stood swaying 
from side to side; when it attempted to walk, it staggered and then swayed 
backward, taking several steps in an effort to catch itself; after this excite- 
ment its head was bent back between its wings. At 4 p. m. the bird was 
found lying on its side and was unable to stand; when it was picked up, 
its head rotated in a circle, sweeping the back and the wings. At 4.15 
about an ounce of clear fluid came from the bird’s mouth. At 5 p. m. 
periods of excitation were observed; the legs were in violent motion as 
in the act of running, and the head shook violently as if the bird were 
